膜联蛋白A1在兔骨髓间充质干细胞体外诱导成骨和成脂早期的表达

摘要

背景:骨髓间充质干细胞分化过程中膜联蛋白A1表达变化的研究报道各有不同看法.目的:分析兔骨髓间充质干细胞在体外诱导成骨和成脂过程中膜联蛋白 A1基因的表达变化.方法:应用全骨髓贴壁法分离培养骨髓间充质干细胞,分别加入含成骨诱导剂、成脂诱导剂及不添加任何诱导剂的培养基进行培养.结果与结论:膜联蛋白A1基因在成骨诱导过程中与未诱导细胞相比有明显下调趋势,差异有显著性意义(P < 0.01),而在成脂诱导剂中则为上升(P < 0.01).成骨诱导剂对细胞生长存在抑制作用并增加细胞凋亡(P < 0.01),而成脂诱导剂对细胞生长与凋亡作用较小(P > 0.05).因此排除了诱导剂对细胞的作用之后,推测膜联蛋白A1基因可能与骨髓间充质干细胞体外向脂肪细胞分化存在一定关系,但尚不能肯定其在成骨分化中的作用.%BACKGROUND: Studies have reported different views on the expression of annexin A1 (ANX A1) in bone marrow mesenchymal stem cells (BMSCs) during their differentiation.OBJECTIVE: To investigate the expression of ANX A1 in rabbit BMSCs during their differentiation into osteoblasts andadipocytes in vitro.METHODS: BMSCs were isolated by whole bone marrow adherence method, and then the cells were cultured in mediawith osteogenic inducer, adipogenic inducer and non-inducer respectively.RESULTS AND CONCLUSION: The expression of ANX A1 mRNA was obviously decreased during the celldifferentiation into osteoblasts compared with those without differentiation (P < 0.01). In contrast, the expression of ANXA1 mRNA was obviously increased in BMSCs cultured in medium with adipogenic inducer (P < 0.01). Osteogenic inducercould inhibit the growth of BMSCs to achicve an increase in apoptosis (P < 0.01), while the adipogenic inducer had littleeffects on cell growth and apoptosis (P > 0.05). ANX A1 may play an important role in regulation of BMSCs differentiationinto adipocytes in vitro without the role of inducer. However, the association with osteogenic differentiation is uncertain.