脊髓损伤亚急性期移植神经干细胞:损伤脊髓5-羟色胺的表达

摘要

背景:5-羟色胺能纤维可以作为评价脊髓损伤后再生的指标之一.目的:观察神经干细胞在脊髓损伤的亚急性期(第7天)脊髓内移植对大鼠受损伤脊髓再生修复的影响.方法:将正常成年SD雌性大鼠分为3组,单纯脊髓全横断组、假手术组(仅仅打开椎板,但不横断脊髓)、神经干细胞移植组.神经干细胞移植组在脊髓全横断后第7天时进行神经干细胞移植,其他两组不移植神经干细胞.结果与结论:神经干细胞移植组瘢痕上1至2节段5-羟色胺的阳性纤维数量多于单纯脊髓全横断组.提示神经干细胞亚急性期移植能部分促进脊髓损伤后脊髓神经的再生.%BACKGROUND: 5-hydroxytryptamine (5-HT) serotonergic fibers are evaluated as one of regenerative indice after spinal cord injury.OBJECTIVE: To study effects of neural stem cells (NSCs) transplantation on the recovery of the rats injured spinal cord in subacute stage (on the 7th day).METHODS: The normal adult SD female rats were divided into 3 groups: merely spinal cord transection group, sham operation group (just opened the lamina, but not transected the spinal cord), NSCs transplantation group. NSCs transplantation group was performed on the 7th day after spinal cord transection, the other two groups were not performed with transplantation.RESULTS AND CONCLUSION: In 1-2 segments above scar of spinal cord, the number of 5-HT positive fibers in NSCs transplantation group was more than that in merely spinal cord transection group. It indicates that NSCs transplantation group in subacute stage can promote the nerve regeneration after spinal cord injury.