绝经后妇女甲状旁腺素基因多态性与骨密度：福州地区的调查

摘要

背景：有研究证实，绝经后妇女骨密度与甲状旁腺素基因有密切关系，但在不同地区人群中结果存在差异性。　　目的：探讨福州地区绝经后妇女甲状旁腺素基因(PTH)BstBⅠ多态性与骨密度的关系。　　方法：用双能X射线骨密度仪检测福州地区150例绝经后妇女的腰椎、股骨颈，大转子和Ward’s三角骨密度，应用PCR-RFLP技术检测甲状旁腺素基因BstBⅠ多态性。　　结果与结论：①甲状旁腺素基因型分布频率为BB型68.8%、Bb型24.1%、bb 型7.1%。等位基因频率为B 81%，b 19%，基因型分布符合Hardy-Weinberg定律。②分析其基因型与骨密度的关系：BB、Bb、bb 3种基因型在股骨颈、大转子、Ward’s三角区4个部位骨密度差异均无显著意义(P>0.05)。甲状旁腺素基因BstBⅠ位点多态性与骨密度间无关联，尚不能作为预测福州地区绝经后妇女发生骨质疏松危险的遗传标志。%BACKGROUND:Studies have shown the bone mineral density of postmenopausal women is closely related to parathyroid hormone. But there are differences in different areas. OBJECTIVE:To investigate the association between BstBⅠ polymorphism of parathyroid hormone gene with bone mineral density in postmenopausal women from Fuzhou area. METHODS:The bone mineral densities of the lumbar spine, femoral neck, trochanter and Ward’s triangle were measured in 150 postmenopausal women by dual energy X-ray absorptiometry. The genotype of parathyroid hormone gene was determined by polymerase chain reaction-restriction fragment length polymorphism. RESULTS AND CONCLUSION:(1) The distribution of parathyroid hormone genotypes were BB genotype 68.8%, Bb 24.1%, and bb 7.1%. The B al elic gene frequencies reached 81%, while b was 19%. The distribution fol owed the Hardy-Weinberg equilibrium. (2) Analysis of the relationship between the genotypes and bone mineral density:There was no significant difference in the bone mineral densities of the lumbar spine, femur, neck, trochanter and Ward’s triangle among the three genotypes (P>0.05). BstBⅠ gene polymorphism of parathyroid hormone gene is not correlated to bone mineral density, and there is no enough evidence to support genotype of parathyroid hormone gene as a genetic marker in predicting the risk of developing osteoperosis in Fuzhou postmenopausal women.