BACKGROUND:Studies have shown that human placenta-derived mesenchymal stem cells with strong proliferative ability have rich sources and can remarkably promote tendon-bone healing after celltransplantation. OBJECTIVE:To observe the effect of human placenta-derived mesenchymal stem cells on tendon-bone healing in a bone tunnel. METHODS:Human placenta-derived mesenchymal stem cells were separated using adherent separation screening method. Thirty 8-week-old male Sprague-Dawley rats were randomly divided into two groups, 15 rats as experimental group and 15 rats as control group. Experimental group were subjected to transplantation of human placenta-derived mesenchymal stem cells and the control group were injected with saline solution. RESULTS AND CONCLUSION:Stem cells, new vessels, and fibrocartilage hyperplasia were observed on the tendon-bone interface with microscope at 2, 4 and 6 weeks after celltransplantation in the experimental group. Biomechanical y, the maximum pul out load in the experimental group was significantly higher than that in the control group at 4 and 6 weeks after celltransplantation (P<0.05). These findings suggest that human placenta-derived mesenchymal stem cells can accelerate early tendon-bone healing in a bone tunnel and strengthen the biomechanical strength.%背景：研究表明，人胎盘来源间充质干细胞来源广泛，增殖能力强，其移植对腱-骨愈合有明显的促进作用。 目的：进一步验证人胎盘来源间充质干细胞对骨-肌腱结合处愈合的影响。 方法：采用贴壁分离筛选法获取人胎盘来源间充质干细胞。30只雄性SD大鼠随机分为2组各15只。建立大鼠骨-肌腱损伤模型，实验组在骨-肌腱结合面注入人胎盘来源间充质干细胞，对照组只注入生理盐水。 结果与结论：实验组人胎盘间充质干细胞移植后2，4，6周显微镜观察在腱骨界面有干细胞存在并有较多血管组织再生，并且有部分纤维软骨增生。在移植后4，6周实验组最大拔出载荷显著高于对照组(P <0.05)。说明人胎盘来源充质干细胞可以促进骨-肌腱结合部的早期愈合，提高其生物力学强度。
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