恒河猴肝移植急性排斥反应中核因子κB p65的表达☆

摘要

BACKGROUND:Nuclear factor-κB as an important nuclear transcription factor, is a converge point for various signal transduction pathways, and participate in the regulation of reactive substances gene expression such as the immune cel proliferation, differentiation and apoptosis. The nuclear factor-κB plays an important role in humoral and cel ular immune. OBJECTIVE:To investigate the relationship between the nuclear factor-κB p65 protein expression and acute rejection in transplanted liver tissue of rhesus monkey. METHODS:The rhesus monkey recipients were randomly divided into two groups:acute rejection group and control group. The acute rejection group did not received anti-rejection treatment after liver transplantation, and the control group was given anti-rejection treatment during and after liver transplantation. The blood samples were col ected at 6, 12, 24 and 72 hours after transplantation, and the automatic biochemical analyzer was used to detect the levels of alanine aminotransferase and total bilirubin. Hematoxylin-eosin staining of transplanted liver tissue was performed to observe the morphological structure and rejection. The degree of rejection was evaluated according to the Banff scoring system, and the expression of nuclear factor-κB p65 in the liver tissue was detected with Western blot. RESULTS AND CONCLUSION:When the acute rejection occurred after liver transplantation, the liver function change was observed after liver histopathological examination, the expression of nuclear factor-κB p65 in the liver tissue was up-regulated, and the degree of acute rejection was increased. In the early stage of acute rejection, the liver function and pathology were changed slightly, while the expression of nuclear factor-κB p65 was significantly increased. The results indicate that the detection of nuclear factor-κB p65 in the transplanted liver tissue has great significance for the early diagnosis of acute rejection after liver transplantation, and the nuclear factor-κB may be the new target for control ing the acute rejection.%　　背景：核因子κB作为一种重要的核内转录因子，是多种信号转导途径的汇聚点，参与机体免疫细胞的增殖、分化及细胞凋亡等多种反应物质基因的表达调控，在体液和细胞免疫中发挥重要作用。目的：探讨恒河猴移植肝组织内核因子κB P65蛋白表达与急性排斥反应的关系。方法：将恒河猴随机分为2组：急性排斥反应组肝移植后不给予抗排斥处理，对照组肝移植过程中及移植后均给予抗排斥处理。分别在移植后6，12，24和72 h 4个时间点收集血标本，全自动生化分析仪测定丙氨酸氨基转移酶、总胆红素，取移植肝脏组织行苏木精-伊红染色观察组织形态结构和排斥反应，根据Banff评分系统判断排斥反应程度，采用Western blot法检测肝脏组织中核因子κB p65的表达。结果与结论：肝移植急性排斥反应发生时肝功能变化滞后于肝组织病理学检查。当急性排斥反应发生时，移植肝组织中核因子κB p65表达上调，急性排斥反应程度也随之加剧。并且在急性排斥反应早期，肝功能、病理学仅有轻微改变时，核因子κB p65表达显著升高。因此移植肝组织中核因子κB p65表达水平的检测对移植后急性排斥反应的早期诊断有重要意义，同时核因子κB可能成为控制移植急性排斥反应的新靶点。