5-氟尿嘧啶纳米微球复合材料填充骨缺损及抗肿瘤作用★

摘要

背景：前期研究成功制备了纳米羟基磷灰石-聚羟基丁酸戊酯/聚乙二醇-5-氟尿嘧啶材料。目的：观察纳米羟基磷灰石-聚羟基丁酸戊酯/聚乙二醇-5-氟尿嘧啶的填充骨缺损和抗肿瘤作用。方法：取72只新西兰兔建立右膝关节 VX2骨肿瘤模型，切除部分胫骨形成骨缺损，且使骨缺损与肿瘤相邻，造模后随机分为实验组与对照组，分别于骨缺损处植入纳米羟基磷灰石-聚羟基丁酸戊酯/聚乙二醇-5-氟尿嘧啶与纳米羟基磷灰石-聚羟基丁酸戊酯/聚乙二醇人工骨。结果与结论：①无机质含量：两组均随时间的延长逐渐降低，尤以实验组明显。②X 射线检查：实验组术后24周材料体积较植入初期变小，肿瘤开始变小。对照植入材料体积始终无明显变化，肿瘤体积进行性增大。③生物力学检测：术后24周，实验组下肢标本最大扭转强度明显高于对照组(P <0.01)。④骨密度检测：术后2-24周，两组均呈先减低后增加趋势，以实验组明显。⑤肿瘤体积变化：术后24周，实验组减小，对照组增加。表明纳米羟基磷灰石-聚羟基丁酸戊酯/聚乙二醇-5-氟尿嘧啶复合材料能很好填充骨缺损并有明显抑制肿瘤生长的作用。%BACKGROUND: Previous studies have successful y prepared nano-hydroxyapatite-poly(3-hydroxybutyrate-hydroxyvalerate)/polyethylene glycol-5-fluorouracil. OBJECTIVE: To discuss the effect of nano-hydroxyapatite-poly(3-hydroxybutyrate-hydroxyvalerate)/polyethylene glycol-5-fluorouracil composite on bone defect repair and against bone tumor. METHODS: Bone tumor models were induced by injection of VX2 carcinoma cellsuspension into the medul ary cavity of right tibias in 72 New Zealand white rabbits through the knee joints. Partial resection of the tibia was performed to form bone defects that were adjacent to bone tumors. After modeling, al the rabbits were randomized into experimental and control groups. Nano-hydroxyapatite-poly(3-hydroxybutyrate-hydroxyvalerate)/polyethylene glycol-5-fluorouracil and nano-hydroxyapatite-poly(3-hydroxybutyrate-hydroxyvalerate)/polyethylene glycol artificial bone were implanted into bone defects, respectively, in the experimental and control groups. RESULTS AND CONCLUSION: (1) Inorganic content was decreased gradual y in the two groups, especial y in the experimental group. (2) X-ray examination: At 24 weeks after implantation, the implant became smal er than that in the early stage after implantation. However, there were no changes in the implant volume in the control group, and tumor volume in the control group was enlarged progressively. (3) Biomechanical detection: At 24 weeks after implantation, the maximal torque of the lower limbs in the experimental group was significantly higher than that in the control group (P < 0.01). (4) Bone mineral density in the two groups was firstly decreased and then increased at 2-24 weeks after implantation. The changes in the bone mineral density of the experimental group were significant. (6) Tumor volume was decreased in the experimental group and increased in the control group at 24 weeks after implantation. These findings indicate that nano-hydroxyapatite-poly(3-hydroxybutyrate-hydroxyvalerate)/polyethylene glycol-5-fluorouracil can perfectly repair bone defects and exert a remarkable effect against tumor growth.