股骨近端微量元素Zn与骨形态发生蛋白7及Stro-1+细胞的相关性：预测髋关节假体寿命

摘要

BACKGROUND:The correlation of zinc with Stro-1+cells and bone morphogenetic protein 7 surrounding the prosthesis may affect the bone fusion and survival rate in hip prosthesis. OBJECTIVE:To analyze the correlation of zinc content with Stro-1+cells and bone morphogenetic protein 7 in proximal femur. METHODS:Bone samples were obtained from the discarded metaphysis region of the proximal femur in 24 patients with primary total hip replacement. Bone marrow mesenchymal stem cells were cultured in vitro. At 14 days after culture, Stro-1+cells in bone marrow mesenchymal stem cells were detected using flow cytometry. Bone morphogenetic protein 7 expression in cellsupernatant was detected using enzyme-linked immunosorbent assay. Zinc content in supernatant was measured using radio-immunity assay. RESULTS AND CONCLUSION:No significant difference in the zinc content was detected in different age groups and different gender groups. Zinc content was positively associated with Stro-1+cells and bone morphogenetic protein 7 expression. The further study of the trace element zinc in proximal femur can predict and intervene the longevity of hip prosthesis.%背景：假体周围微量元素Zn的含量与骨形态发生蛋白7、Stro-1+细胞相关性可能影响髋关节假体的骨融合与生存率。　　目的：分析股骨近端微量元素Zn与骨形态发生蛋白7、Stro-1细胞的相关性。　　方法：24例初次髋关节置换患者，取术中股骨矩开槽时废弃骨块，提取细胞后体外培养骨髓间充质干细胞。培养14 d采用流式细胞仪检测骨髓间充质干细胞中的Stro-1+细胞，酶联免疫吸附测定试剂盒检测培养细胞离心上清液中骨形态发生蛋白7的表达，放射免疫荧光法检测培养细胞离心上清液中Zn含量。　　结果与结论：Zn含量在不同年龄组及不同性别组差异无显著性意义；Zn与Stro-1+、骨形态发生蛋白7正相关联系。关于股骨近端微量元素Zn的进一步研究可以用来预测和干预髋关节假体的寿命。