背景：有研究表明不同的纳米表面形貌对细胞的生物活性具有不同的影响，但纳米网状结构至今少有报道。目的：观察纳米网状结构对骨髓间充质干细胞生物活性的影响。方法：实验利用碱热处理法，制备获得纳米网状的表面形貌材料，利用纯钛作为对照组材料，将两种材料分别与骨髓间充质干细胞共培养，利用扫描电镜及免疫荧光分别观察细胞形态及细胞骨架，再利用培养不同时间后细胞的吸光度值依次检测细胞早期黏附、增殖及成骨分化情况。结果与结论：两组材料与细胞共培养30，60，120 min时，纳米网状结构组材料表面黏附的细胞数量明显多与纯钛。共培养第1，3，5天时，纳米网状结构组材料均可以明显促进细胞增殖，吸光度值明显高于纯钛组(P <0.05)。两组材料与细胞成骨诱导培养14 d后，与纯钛组相比，纳米网状结构组材料的碱性磷酸酶值明显升高(P <0.05)，其材料表面的细胞形态及细胞骨架结构均较好。结果证实，与传统的纯钛材料相比，纳米网状结构可以更好的调节骨髓间充质干细胞的生物活性。%BACKGROUND:Many studies have shown that different nanostructures produce different influences on cellbioactivity, but the nanonetwork structure is not reported yet. OBJECTIVE:To study the influence of the nanonetwork topography on the bioactivity of bone marrow mesenchymal stem cels. METHODS:The nanonetwork topography was fabricated on biomedical titanium surface by alkali-heat treatment, and pure titanium served as control group. Bone marrow mesenchymal stem cels were co-cultured with the above two types of samples. cellmorphology and cytoskeleton were observed using scanning electron microscope and immunofluorescence method. The celladhesion, proliferation and osteogenic differentiation were detected by measurement of absorbance values at different culture time. RESULTS AND CONCLUSION: The nanonetwork topography had significant advantage on the number of adherent cels at 30, 60 and 120 minutes of co-culture. The cellproliferation was significantly accelerated by the nanonetwork topography at days 1, 3, 5 of co-culture, and the absorbance values in the nanonetwork group were significantly higher than those in the pure titanium group (P < 0.05). The alkaline phosphatase activity in the nanonetwork group was also significantly higher than that in the pure titanium group at 14 days of osteogenic induction (P < 0.05). The cellshape and cytoskeleton on the nanonetwork surface were better than those on the titanium surface. These findings indicate that the nanonetwork topography has better effects on cellbioactivity compared with pure titanium.
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