人卵巢颗粒细胞分离培养方法的改进

摘要

BACKGROUND:To build up an effective method of isolating and culturing granule cels is a pivotal step to enhance fertilization-embryo transfer rate. Current studies mainly focus on the isolation methods of human ovarian granulosa cels rather than cel counting, purity and subsequent growth. OBJECTIVE: To establish the effective methods of isolating, purifying and culturing human ovarian granulosa cels in vitro. METHODS: Folicular fluid was harvested from women undergoing fertilization-embryo transfer procedures. Human ovarian granulosa cels were obtained from the folicular fluid by lysis treatment, precipitation method or density gradient centrifugation. Granulosa cel mucus masses were digested with type I colagen enzyme or hyaluronidase and then cultured in the culture medium with or without autologous folicular fluid. RESULTS AND CONCLUSION: Lysis treatment yielded the largest amount of granulosa cels compared to the precipitation method and density gradient centrifugation (P > 0.05,P < 0.05, respectively). Cels prepared by the three methods showed the same cel viability. After 24 hours of culture, the precipitation method obtained the largest amount of adherent granulosa cels (P < 0.05); and the density gradient centrifugation obtained the least amount of cels (P < 0.05). Compared with type I colagen enzyme, hyaluronidase took less time to digest the cels thoroughly. Autologous folicular fluid could promote the growth and survival of granulosa cels. These findings indicate that the precipitation method, though time-consuming, can obtain the highest cel viability and harvested the largest amount of granulosa cels after culture; hyaluronidase is more suitable for digesting granulosa cel mucus mass than type I colagen enzyme; autologous folicular fluid added into the culture medium is more conducive to granulosa cel growth.%背景：建立分离培养颗粒细胞快速有效的方法也是提高体外受精-胚胎移植成功率关键的一步。虽然目前文献中有较多关于人卵巢颗粒细胞分离方法的报道，但在细胞数量、纯度及后续生长等方面不尽人意。目的：建立有效的分离提纯、体外培养人卵巢黄素化颗粒细胞的方法。方法：收集体外受精-胚胎移植取卵时的卵泡液，用裂解法、沉淀法、密度梯度离心法分离，Ⅰ型胶原酶或透明质酸酶消化颗粒细胞黏液团并接种在培养皿中进行培养，培养液中加入或不加自体卵泡液。结果与结论：用裂解法得到的颗粒细胞数较沉淀法和密度梯度离心法得到的细胞数多(P >0.05，P <0.05)；3种方法提取的颗粒细胞活性比较无明显差异；培养24 h后沉淀法贴壁细胞数最多(P <0.05)，而密度梯度离心法贴壁细胞数最少(P <0.05)；透明质酸酶消化颗粒细胞较Ⅰ型胶原酶耗时少且消化彻底；加入自体卵泡液能够促进颗粒细胞生长和存活。结果证实，沉淀法提取颗粒细胞虽然耗时长，但培养的细胞存活率高、培养后收获的细胞数多；透明质酸酶较Ⅰ型胶原酶更适合消化颗粒细胞黏液团；在培养液中加入自体卵泡液更有益于颗粒细胞生长。