背景：目前研究发现800 m跑的代谢特征不是十分明晰，为800 m的运动训练造成了很大的阻碍。目的：了解800 m跑项目的物质和能量代谢特征，提高800 m跑训练的科学化水平，验证基于核磁共振的尿液代谢组学技术在运动训练监测方面的可行性。方法：对某高校运动训练专业7名中跑2级运动员进行一次极限强度800 m跑，结合人体代谢数据库(HMDB)等数据库，对运动前、后的尿液进行代谢组学相关分析。结果与结论：OPLS-DA模型显示运动前和运动后样本的代谢模式呈现簇类分布。造成这一变化的特征性代谢物主要有亮氨酸、乳酸、肌酐、琥珀酸、次黄嘌呤、腺苷等代谢物。通过分析可以推断出800 m跑是以糖酵解供能为主、磷酸原供能和有氧氧化供能共同参与的运动项目，800 m跑后运动员氨基酸代谢、甲烷代谢等代谢过程较为旺盛，而且在800 m跑的过程中会出现一定程度的氧化应激。结果说明基于核磁共振的尿液代谢组学技术有无创性、灵敏度高、样品需求量少、处理简单等优势。%BACKGROUND:Metabolic characteristics of the 800 meters race is not very clear, which creates a lot of obstacles for the exercise training of 800 meters race. OBJECTIVE: To investigate the characteristics of material and energy metabolism of 800 meters race, to improve the scientific training of the 800 meters race and to verify the feasibility of nuclear magnetic resonance-based urine metabonomics in sports training monitoring. METHODS: Seven middle distance athletes were subject to an 800 meters race at ultimate intensity, and their urine metabonomics analysis was conducted in combination with the Human Metabolism Database and other databases before and after exercise. RESULTS AND CONCLUSION:OPLS-DA model showed that the metabolic samples before and after movement presented the cluster-like distribution. The change was mainly caused by the folowing metabolites, including leucine, lactic acid, creatinine, succinic acid, hypoxanthine, and adenosine. Through the analysis we could infer that the 800 meters race is a sport event mainly dependent on glycolysis for energy supply, working together with raw phosphate and aerobic oxidation for energy supply. The amino acid metabolism and methane metabolism of the athletes were more vigorous after 800 meters running, and moreover, there were certain oxidative stress during the 800 meters race. These findings indicate that nuclear magnetic resonance-based urine metabonomics takes the advantages of invasive, high sensitivity, less sample requirement, simple processing.
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