背景：脐血与骨髓来源的间充质干细胞在一定诱导条件作用下均具有多向分化的能力。目的：比较脐血与骨髓来源间充质干细胞向成骨细胞诱导分化能力的差异。方法：采用密度梯度法分离培养人脐血间充质干细胞和骨髓间充质干细胞，当细胞汇合90%后胰蛋白酶消化传代，取第3代脐血间充质干细胞和骨髓间充质干细胞，以细胞密度为8×104/孔接种，当细胞达80%融合时，加入含体积分数为10%胎牛血清、0.1μmol/L地塞米松、50μmol/L维生素C、10 mmol/Lβ-甘油磷酸钠的低糖型DMEM成骨细胞诱导液培养。结果与结论：两种来源间充质干细胞的形态和生物学特性无明显差异，细胞表面均强表达CD44，CD29，不表达 CD34；两者均具有向成骨细胞诱导分化的潜能，稳定表达了成骨细胞标志性的产物碱性磷酸酶、骨矿化结节，而且两种细胞的成骨活性差异无显著性意义。结果表明脐血和成人骨髓来源间充质干细胞向成骨细胞诱导分化能力相似，均可作为骨组织工程的种子细胞。%BACKGROUND:Mesenchymal stem cels isolated from cord blood and bone marrow have multi-directional differentiation ability under a certain condition of induction. OBJECTIVE:To compare the difference of differentiation of umbilical cord blood and bone marrow mesenchymal stem cels into osteoblasts. METHODS:Human umbilical cord blood and bone marrow mesenchymal stem cels were isolated and cultured by density gradient method. When reached 90% confluency, mesenchymal stem cels were digested by trypsin for subculture. At the third passage, umbilical cord blood mesenchymal stem cels and bone marrow mesenchymal stem cels at 8×104/wel were incubated. When reached 80% confluency, cels were treated with low-glucose DMEM supplemented with 10% fetal bovine serum, 0.1 μmol/L dexamethasone, 50 μmol/L vitamin C and 10 mmol/L β-sodium glycerophosphate. RESULTS AND CONCLUSION:There was no significant difference in morphology and biological properties of the two kinds of mesenchymal stem cels. Cels were highly expressed CD44, CD29, but did not express CD34. They had the ability to differentiate into osteoblasts, which had a positive staining for known markers: alkaline phospatase and calciumin vitro mineralization. There was no significant difference in the activity of osteoblasts of two kinds of cels. Results verify that umbilical cord blood and adult bone marrow mesenchymal stem cels can be induced into osteoblasts with a similar ability, and they can be used as seed cels for bone tissue engineering.
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