人参皂苷Rg1与神经干细胞共培养的促增殖和保护作用

摘要

BACKGROUND:Chinese herb extracts can restore and protect the nervous system of rats through intervention of neural stem cels. OBJECTIVE:To explore the role of ginsenosides Rg1 in the proliferation and protection of neural stem cels. METHOD:Sprague-Dawley rats at pregnant 19 days were dissected to take out fetal rats, and then the hippocampal tissues from fetal rats were isolated to extract neural stem cels. Neural stem cels were co-cultured with DMEM/F12 medium containing 50 g/L ginsenosides Rg1 as intervention group, with DMEM/F12 medium as blank control group, and with DMEM/F12 containing 0.64% phenol as positive control group, respectively. MTT assay was used to detect the proliferation of neural stem cels in each group, and western blot method to detect the protein expression of brain-derived neurotrophic factor and transforming growth factor-β in neural stem cels. RESULTS AND CONCLUSION:Rat neural stem cels were round single cels with clear border at early period after isolation but at 2 days after inoculation, the cels were adherent and aggregated into smal cel spheres. Compared with the blank control group, the proliferative rate of neural stem cels was significantly increased in the ginsenosides Rg1 group (P < 0.05), but decreased in the positive control group (P < 0.05). Compared with the blank control group, in the ginsenosides Rg1 group, the expression of brain-derived neurotrophic factor was elevated, and the expression of transforming growth factor-β was reduced, indicating ginsenosides Rg1 has a certain effect to promote the proliferation of neural stem cels as wel as to protect the neural stem cels.%背景：研究表明传统中药提取物对大鼠神经干细胞进行干预，能够反映其对神经系统的恢复和保护作用。目的：探讨人参皂苷Rg1对神经干细胞的增殖和保护作用。方法：将妊娠19 d左右的SD大鼠进行解剖取出胎鼠，然后分离海马组织提取神经干细胞，与含50 g/L人参皂苷Rg1的DMEM/F12培养基共培养作为人参皂苷Rg1组，正常DMEM/F12培养基培养的神经干细胞作为空白对照组，与含0.64%苯酚的DMEM/F12培养基共培养作为阳性对照组，MTT法检测各组神经干细胞的增殖情况，Western-blot方法检测各组神经干细胞脑源性神经营养因子和转化生长因子β蛋白的表达。结果与结论：神经干细胞分离初期在镜下为圆形单个细胞，细胞的边缘比较清楚，接种2 d后，细胞贴壁并形成小的细胞球。人参皂苷Rg1组大鼠神经干细胞增殖率显著高于空白对照组(P<0.05)，阳性对照组神经干细胞增殖率显著低于空白对照组(P<0.05)。与空白对照组相比，人参皂苷Rg1组大鼠神经干细胞脑源性神经营养因子蛋白表达显著升高，转化生长因子β蛋白表达显著下降，以上结果提示人参皂苷Rg1对大鼠神经干细胞具有一定的促进增殖和保护作用。