BACKGROUND:An intensivestudy on ovarian cancer A2780 cels contributes to modify treatment strategies targeting ovarian cancer stem cels and enhance survival rate of ovarian cancer patients. OBJECTIVE:To investigate the effects of umbilical cord blood mesenchymal stem cels (UC-MSCs) transplantation combined with paclitaxel on proliferation, apoptosis, and invasion of A2780 human ovarian cancer celsin vitro. METHODS:A2780 human ovarian cancer cels were assigned into three groups: blank control group (routine cel culture),UC-MSCs group (addition of suspension containing 1×106UC-MSCs) and combined treatment group (combined addition of suspension containing 1×106UC-MSCs and 250μL of 1μmol/L paclitaxel solution). CD133+antigen expression, proliferation, apoptosis, and invasion were determined by immunofluorescent staining, flow cytometry, TUNEL staining, and Transwel chamber invasion assay, respectively. RESULTS AND CONCLUSON:CD133+antigen expression intheUC-MSCs group and combined treatment group was significantly decreased compared withtheblank control group (P< 0.05). Proliferation and invasion abilities were significantly decreased, while apoptosis cel number was significantly increased inthecombined treatment group compared with the other two groups (P< 0.05). These findings indicate that the combined treatment of UC-MSCs transplantation and paclitaxel can inhibit proliferation and invasion and promote apoptosisof A2780 human ovarian cancer cels.%背景：深入进行卵巢癌肿瘤干细胞的研究，有助于以卵巢癌肿瘤干细胞为靶点，改进卵巢癌的治疗策略，提高卵巢癌的生存率。 目的：探讨脐血间充质干细胞联合紫杉醇对卵巢癌A2780细胞增殖、凋亡、侵袭能力的影响。 方法：将人卵巢癌 A2780细胞分为3组：空白对照组(常规培养细胞)、脐血间充质干细胞组(加入1×106个脐血间充质干细胞悬液)、联合组(加入1×106个脐血间充质干细胞悬液的同时加入1μmol/L紫杉醇溶液250μL)。采用免疫荧光法检测卵巢癌A2780细胞CD133+抗原表达，流式细胞仪、TUNEL染色法、Transwel 小室侵袭实验检测卵巢癌A2780细胞的增殖、凋亡和侵袭能力。 结果与结论：①空白对照组CD133+阳性抗原表达量很多，明显高于脐血间充质干细胞组及联合组，差异有显著性意义(P<0.05)；②联合组细胞增殖能力、侵袭能力明显减弱，凋亡细胞数明显增多，与其他两组比较差异有显著性意义(P <0.05)；③结果表明，脐血间充质干细胞联合紫杉醇能抑制卵巢癌A2780细胞的增殖、侵袭，并促进其凋亡。
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