Objective The ex vivo cytotoxicity of Ceramicrete-based root-end filling materials were tested to lay the experimental foundation for clinical application.Methods The mitochondrial succinate dehydrogenase activities of a rat osteoblast-like cell line ( Ros 17/2.8 ) to ceramicrete-based root-end filling materials were examined using methyl thiazolyl tetrazolium(MTT) assay.Ultrastructural change of cells was observed under Transmission electron microscopy( TEM ).Set Ceramicrete-based materials immersed in simulated body fluid (SBF) for 24 h were examined with scanning electron microscopy ( SEM ).Results Ceramicrete-based root-end filling materials exhibited severe toxicity in the first stage,relative cell proliferation rates ranged from 0.95％ to 2.45％.After which toxicity decreased rapidly over time,mild toxicity was exhibited in the second stage with cell proliferation rates ranging from 73.65％ -88.62％.Nontoxicity was achieved in the sixth stage with cell proliferation rates ranging from 91.53％ -97.25％.TEM of cells exposed to the Ceramicretes for different time-periods revealed variable degrees of cell injury that were consistent with the MTT results.SEM of the set Ceramicrete-based materials immersed in SBF revealed the presence of needle-shaped crystallites over the material surfaces.Conclusions The experimental Ceramicrete-based materials exhibited good biocompatibility and potential bioactivity.%目的 体外评估Ceramicrete根管倒充填材料的细胞毒性和生物学活性,以期为临床应用提供实验室基础.方法 采用甲基噻唑基四唑(MTT)实验评估Ceramicrete根管倒充填材料对大鼠成骨样细胞ROS 17/2.8的细胞毒性.透射电子显微镜观察不同阶段细胞超微结构的形态学变化.扫描电子显微镜观察Ceramicrete根管倒充填材料浸泡于模拟体液中24 h的形态学变化.结果 第1阶段ROS17/2.8细胞表现为重度毒性,细胞相对增值率为0.95％～2.45％；随后细胞毒性呈下降趋势,第2阶段为轻度毒性,细胞相对增值率为73.65％ ～ 88.62％；第6阶段无细胞毒性,细胞相对增值率为91.53％ ～ 97.25％.透射电镜下细胞的超微结构变化显示细胞损伤程度与MTT实验一致；扫描电镜下观察到在模拟体液中Ceramicrete倒充填材料表面有成簇的针状晶体生长.结论 Ceramicrete 根管倒充填材料具有良好的生物相容性和潜在的生物学活性.
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