灵芝多糖对长期大强度运动小鼠巨噬细胞活性及功能的影响

摘要

目的:研究灵芝多糖(GLP)对长期大强度运动小鼠巨噬细胞活性及功能的影响.方法:实验小鼠50只随机分为盐水+对照组、盐水+运动组、低剂量GLP+运动组、中剂量GLP+运动组、高剂量GLP+运动组,每组10只.3个GLP组每天分别按50 mg/kg/日GLP(低剂量)、100 mg/kg/日GLP(中剂量)、200 mg/kg/日GLP(高剂量)灌胃.盐水+对照组和盐水+运动组每天灌胃等体积生理盐水,共4周.对照组不运动,4个运动组小鼠每天进行1次45 min负重5%游泳训练,每周6次,为期4周.测定4周后各组小鼠巨噬细胞分泌的NO、IL-1β水平和细胞内GSH及吞噬率、吞噬指数.结果:4周运动后盐水+运动组小鼠腹腔巨噬细胞分泌的NO、IL-1β水平显著下降.盐水+运动组小鼠腹腔巨噬细胞吞噬率显著下降.盐水+运动组小鼠腹腔巨噬细胞吞噬指数呈现下降趋势.中剂量GLP+运动组小鼠腹腔巨噬细胞分泌的NO、IL-1β水平显著高于盐水+运动组,高剂量GLP+运动组显著高于盐水+运动组和低剂量GLP+运动组,中、高剂量GLP+运动组小鼠细胞内GSH水平显著低于盐水+运动组和低剂量GLP+运动组,随GLP剂量增加GSH逐渐下降.低、中、高剂量GLP+运动组小鼠腹腔巨噬细胞吞噬率显著高于盐水+运动组,随GLP剂量增加巨噬细胞吞噬率逐渐提高.中剂量GLP+运动组小鼠腹腔巨噬细胞吞噬指数显著高于盐水+运动组和低剂量GLP+运动组.结论:GLP对大强度运动小鼠腹腔巨噬细胞吞噬功能及分泌IL-1β、NO具有明显的促进作用.%Objective To investigate the effects of ganoderma lucidum polysaccharide (GLP) on macrophages activity and functions in long-term high intensity exercise mice.Methods 50 experimental mice were randomly divided into 5 groups: saline water + control group(S+C), saline water + training group(S+T), low dose GLP + training group(LG+T), medium dose GLP + training group(MG+T), high dose GLP + training group(HG+T), 10 mice in each group.Different dosage of GLP were supplemented in GLP groups, 50mg/kg/day for low dose group, 100mg/kg/day for medium dose group and 200mg/kg/dayfor high dose group.S+C group and S+T group were treated with same volume of saline water per day, for 4 week.Four weeks load swimming training program with 5％ body weight were conducted in training groups, 45mins per day, 6 days per week.After 4 weeks, the macrophage secreting NO, IL-1βand intracellular GSH, phagocytic percentage, phagocytic index of mice in each group were measured.Results After 4 weeks exercise, the macrophage secreting NO, IL-1β of peritoneal cavity in S+T group decreased obviously.The macrophage phagocytic percentage of peritoneal cavity in S+T group decreased obviously.The macrophage phagocytic index in S+T group showed decrease.Macrophage secreting NO, IL-1β in MG + T was obviously higher than that in S+T group, Macrophage secreting NO, IL1β in HG+T were obviously higher than that in S+T group and LG+T group.The GSH in medium and high dose GLP + T groups was obviously less than that in S+T and LG+T group, GSH decreased with the increase of GLP dosage.The macrophage phagocytic percentage of peritoneal cavity in low, medium and high dose GLP+T group were obviously higher than that in S+T group, the macrophage phagocytic percentage increased with increase of GLP dosage.Macrophage phagocytic index of peritoneal cavity in MG+T group was obviously higher than that in S+T and LG+T group.Conclusion GLP obviously promoted peritoneal macrophage phagocytic function and IL-1β' s and NO' s secreting in high intensity exercise mice.