Objective To study the effect of curcumin on the IL-10-induced IL-8,sICAM-l,and NO and PGE2 productions in articular chondrocytes of rabbit. Methods IL-ip-induced IL-8,sICAM-l, and NO and PGE2 productions in rabbit chondrocytes were measured respectively. The results were analyzed by statistical method. Results As compared with the blank control, rhIL-1β -induced IL-8, sICAM-l.and NO and PGE2 expressions increased respectively. Conclusion IL-1β significantly increases the concentrations of IL-8,sICAM-l and NO and PGE2,and curcumin can dose-dependently decrease the concentrations of IL-8,sICAM-l,and NO and PGE in the chondrocytes supernatant in vitro.%目的:研究姜黄素对兔软骨细胞体外诱导分泌白介素8 (IL-8)、sICAM-1和一氧化氮(N0)、前列腺素(PGE2)的影响.方法:将10 μg/L浓度的白介素1β(IL-1β)分别与低、中、高浓度的姜黄素共育于兔关节软骨细胞,用IL-1β体外诱导兔软骨细胞分泌IL-8、sICAM-1和NO、PGE2,采用ELISA法检测兔软骨细胞分泌IL-8和sICAM-1的量,分别采用硝酸还原酶法和ELISA酶联免疫竞争法测定细胞上清液NO含量和PGE2含量.结果:IL-1β(10 μg/L)组兔软骨细胞分泌的IL-8和sICAM-1较空白对照组升高,姜黄素对IL-1β诱导分泌的IL-8和sICAM-1起抑制作用,同时其对IL-1β诱导产生的NO、PGE2也起抑制作用.结论:姜黄素可显著抑制IL-1β刺激兔软骨细胞分泌促炎症因子IL-8和sICAM-1,并可显著抑制NO、PGE2途径,对软骨细胞具有一定的保护功能.
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