利用染色体片段置换系定位水稻赖氨酸含量的QTL

摘要

以籼稻品种9311为受体、粳稻品种日本晴为供体构建95个染色体片段置换系,对水稻控制赖氨酸含量的QTL进行了定位.结果显示,共有7个染色体片段置换系(chromosome segment substitution line,CSSL)的稻米赖氨酸含量与亲本9311差异显著.利用代换作图法共鉴定了4个与赖氨酸含量相关的QTL,分别位于水稻第8、9和12染色体上.其中qHLY8和qHLY9.2来自高赖氨酸含量籼稻品种9311,正向加性效应百分率分别为9.6％和8.5％；而qHLY9.1和qHLY12则来自低赖氨酸含量梗稻品种日本晴,负向加性效应百分率分别为-16.0％和-21.3％.%A total of 95 chromosome segment substitution lines (CSSLs) derived from 9311 and Nipponbare was usedrnto detect quantitative trait loci (QTLs) controlling the lysine content of rice. The result showed that the lysine contentrnof seven CSSLs differed from that of 9311. Totally four QTLs for the lysine content of rice were preliminary mappedrnon the chromosomes 8, 9 and 12 by substitution mapping. Among these QTLs, qHLY8 and qHLY9. 2 were bothrncontributed by the indica variety 9311 with high lysine content with the positive effect percentage being 9. 6% and 8.rn5%, respectively; while qHLY9. 1 and qHLY12 were derived from the japonica variety Nipponbare with low lysinerncontent with the negative effect percentage of - 16. 0% and -21. 3% , respectively.