云南野生稻抗稻瘟病Pi-ta基因的检测及分析

摘要

A total of 1 6 accessions of wild rice from different regions of Yunnan Province were used for PCR amplifica-tion with Pi-ta gene specific primers.The amplified PCR products were cloned into Topo cloning vector,and se-quenced.Among 1 6 accessions,the identical sequence fragment to the resitance Pi-ta gene was amplified separately from 7 accessions.The Pi-ta allele in one accession of Oryza rufipogon with purple stem from Jinghong contains an i-dentical DNA sequence in the coding region to the original Pi-ta resistance gene.It suggested that Yunnan might be one of the origins of the Pi-ta gene.%以16个不同来源的云南野生稻作为供试材料,用抗稻瘟病 Pi-ta 基因特异引物(KG2/KG4)进行 PCR 扩增,并对PCR 产物进行克隆、测序及分析。结果表明,16个野生稻中7个品种持有抗稻瘟病 Pi-ta 基因,其中,来自云南景洪的紫秆普通野生稻中 Pi-ta 基因的编码区与原始型的抗稻瘟病 Pi-ta 基因的 DNA 序列完全相同。可见,云南可能是抗稻瘟病基因Pi-ta 的起源地之一。