Objective To investigate the influence of PM2.5 tracheal exposure on organ development in mice and the interventive effect of quercetin during gestation and lactation.Methods 60 SPF Pregnant mice of Institute of Cancer Research,which provided by Peking University of Health Science Center,were randomly divided into 5 groups with 12 mice each group,including control group,PM2.5 model group,and 3 quercetin intervention groups (Low,middle,and high dose group).Pregnant mice in all groups but the control group,were exposed to PM2.5 suspension (15.0mg/kg) by intratracheal instillation on gestational day 3,6,9,12,15 and lactation day 2,5,8,11,14,17 respectively.The control dams were administered with same amount of suspension from extracts of " blank" filter on the same time points.Meanwhile,each dam was given 0.15％ carboxymethylcellulose sodium (CMC) in control group and PM2.5 model group,and different doses (50,100,200 mg/kg) of quercetin in the 3 quercetin intervention groups by garage once a day during gestational and lactation.Quercetin was suspended in 0.15％ CMC.All dams were allowed to natural labor and numbers of offspring and gender were recorded.On lactation day 3,6 offspring per litter with each sex of 3 were kept.One male and one female offspring from each litter were sacrificed on lactation day 3,10,21,35,and organs (heart,liver,spleen,lung,kidney) were weighted and the intestinal tract length was measured.Results There were significant differences between PM2.5 model group and control group with the weight and coefficient of major organs at the four time points,especially for heart,spleen and kidney.Low and middle-dosed quercetin intervention group showed protective effect compared to PM2.5 model group.In addition,the small intestine length in PM2.5 model group was significantly shorter than control group in lactation day 10,21,and 35.Conclusion Mice exposed to PM2.5 during gestation and lactation could reduce the weight and coefficient of major organs,including heart,spleen and kidney and small intestine length of the offspring.50 and 100mg/kg of quercetin administration during gestation and lactation might protect the dams against the adverse effects.%目的 探讨孕期和哺乳期PM2.5气管暴露对子代小鼠主要脏器发育的影响及槲皮素的干预效果.方法 将北京大学医学部实验动物科学部提供健康成年SPF级ICR孕鼠60只随机分为空白对照组、PM2.5模型对照组和低、中、高三个槲皮素干预组,每组12只.于孕3、6、9、12、15天和产后2、5、8、11、14、17天分别进行气管滴注,除空白对照组外,其余各组均滴注PM2.5溶液(15 mg/kg),空白组给予相应剂量的干净滤膜洗脱液;各组孕鼠于孕期和哺乳期每天灌胃,空白对照组和PM2.5模型组给予0.15％羧甲基纤维素钠灌胃,槲皮素三个干预组分别予以50 mg/kg、100 mg/kg、200 mg/kg的槲皮素羧甲基纤维素钠混悬液灌胃.所有孕鼠自然生产,并记录生产仔鼠只数及性别.于产后3天调窝至每窝6只仔鼠,雌雄各半,于产后21天处死母鼠,并于生后3天、10天、21天、5周每窝仔鼠分别处死一雌一雄,摘取脏器(心、肝、脾、肺、肾)并称重,并计算脏器系数,同时测量肠道长度.结果 四个时间点仔鼠的脏器重量和系数模型组和空白组之间差异有统计学意义,尤其表现为心、脾、肾,而槲皮素低、中剂量组与PM2.5模型组相比表现出一定的干预作用.PM2.5模型组仔鼠生后10天、21天及5周的小肠长度显著小于空白对照组(P＜0.05). 结论 孕期及哺乳期PM2.5气管暴露对子代小鼠的心、脾、肾重量和脏器系数产生影响,并且使得子代小鼠小肠长度变短.孕期和哺乳期进行50 mg/kg和100 mg/kg的槲皮素干预可以在一定程度上减少PM2.5的上述发育损害.
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