GAS6基因344-35C/T多态性与脑梗死的相关性研究

摘要

Objective To investigate the correlations of the single nucleotide polymorphism (SNP) 344-35C/T of growth arrest-specific gene 6 (GAS6) gene and cerebral infarction in Han population in the western Guangdong province. Methods The restriction fragment length polymorphism (PCR-RFLP) was used to determine the 344-35C/T polymorphism in the intron region of GAS6 gene in the case group (n=180) and healthy control group (n=150). Results There was no significant association between GAS6-344-35C/T and cerebral infarction (P＞0.05). After stratified by gender in chi-square test in women population, the frequency of C allele was significantly higher in cases (77.9％)than that in controls (66.4％) (P=0.040); The frequency of the CC genotype was significantly higher in cases (61.8％) than that in controls (43.1％) (P=0.036). Conclusion In Han women in western Guangdong province, C allele of GAS6-344-35C/T polymorphism is a risk factor of cerebral infarction, CC genotype is a susceptible genotype of cerebral infarction.%目的 探讨粤西地区汉族人群中生长停滞特异性基因6(GAS6基因)内含子SNP344-35C/T位点的单核苷酸多态性(SNP)与脑梗死的相关性.方法 应用聚合酶链反应.限制性内切酶片段长度多态性分析方法(PCR-RFLP)检测180例脑梗死患者和150例健康对照的GAS6基因内含子SNP344-35C/T的基因型.结果 GAS6基因内含子SNP344-35C/T基因型及等位基因频率在病例组与对照组之间无显著性差异(P>0.05),经性别分层分析发现,女性C等位基因频率(77.9%)高于对照组(66.4%)(P=0.040);CC基因型频率(61.8%)高于对照组(43.1%)(P=0.036).结论 GAS6基因内含子SNP344-35C/T位点与粤西地区汉族女性脑梗死有关,C等位基因是女性脑梗死的危险因素,CC基因型为女性脑梗死的易感基因型.