乙酰左旋肉碱对急性脊髓损伤大鼠细胞自噬、凋亡及运动功能的影响

摘要

目的：旨在检测乙酰左旋肉碱(ALC)对大鼠急性脊髓损伤(SCI)后微管相关蛋白1轻链3(LC3)-Ⅱ、细胞凋亡及运动功能的影响。方法成年雌性Sprague-Dawley大鼠36只随机分为假手术组(Sham组)、单纯脊髓损伤组(SCI组)、ALC治疗组，每组12只。Allen法建立大鼠T10脊髓损伤模型。损伤后3 d行BBB运动评分；取脊髓组织，Western blotting和免疫荧光标记技术检测LC3-Ⅱ表达，TUNEL法观察细胞凋亡。结果与Sham组相比，SCI组LC3-Ⅱ明显升高(P<0.01)，细胞凋亡显著增加(P<0.001)， BBB评分显著降低(P<0.001)；与SCI组相比，ALC组LC3-Ⅱ显著升高(P<0.001)，细胞凋亡显著降低(P<0.001)，BBB评分明显提高(P<0.01)。结论 ALC可能通过增强大鼠脊髓损伤后细胞自噬，减少细胞凋亡，促进大鼠运动功能恢复。%Objective To observe the effects of acetyl-l-carnitine (ALC) on autophagy, apoptosis and motor function after acute spinal cord injury (ASCI) in rats. Methods Thirty-six adult female Sprague-Dawley rats were randomly divided into sham operation group (Sham group, n=12), simple spinal cord injury group (SCI group, n=12), ALC treatment group (ALC group, n=12). Spinal cord injury model at the level of T10 segment was established using Allen's method. They were assessed with Basso-Beattle-Bresnahan (BBB) scale three days after injury. Then the rats were sacrificed, and the expression of microtubule associated protein 1 light chain 3 (LC3)-II in spinal cord was detect-ed with Western blotting and immunofluorescent labeling, and the number of apoptotic cells were assessed with TUNEL staining. Results The expression of LC3-II and the number of apoptotic cells increased in SCI group compared with those in Sham group (P<0.01), while the BBB score decreased (P<0.001). The expression of LC3-II increased and the number of apoptotic cells decreased in ALC group compared with those in SCI group (P<0.001), while the BBB score increased (P<0.01). Conclusion ALC may promote autophagy, and inhibit apopto-sis to improve the locomotor function after ASCI.