超短波促进巨噬细胞表型转化并抑制大鼠脊髓损伤后炎症反应的作用

摘要

目的 探讨超短波治疗对巨噬细胞表型转化及大鼠脊髓损伤的保护作用.方法 Sprague-Dawley大鼠96只随机分为假手术组、模型组和超短波组,每组32只.模型组和超短波组采用改良Allen法构建大鼠脊髓损伤模型,超短波组于术后第1天起于损伤部位行小剂量超短波治疗.采用BBB评分评估各组大鼠运动功能;HE染色评估各组脊髓空洞大小;免疫组织化学染色、实时PCR及Western blotting法检测各组脊髓组织中诱导型一氧化氮合酶(iNOS)、精氨酸酶-1(Arg-1)和肿瘤坏死因子-α (TNF-α) mRNA及蛋白表达.结果 与模型组比较,超短波组BBB评分升高(P<0.05),iNOS和TNF-α表达减少,Arg-1表达增加(P<0.05).结论 超短波治疗可促进脊髓损伤周围巨噬细胞从M1型向M2型转化,抑制炎症反应并促进脊髓损伤后修复.%Objective To explore the effect of ultrashort wave on macrophage polarization and inflammation response after spinal cord injury in rats. Methods A total of 96 Sprague-Dawley rats were randomly divided into sham group, model group and ultrashort wave group, with 32 rats in each group. The spinal cord injury model was established by modified Allen's method in the model group and the ultrashort wave group. The ultrashort wave group received ultrashort wave since the first day after modeling. BBB score was applied to evaluate the locomotion recovery. HE staining was used to as-sess the injury area, while immunochemistry, real-time PCR and Western blotting were applied to measure the mRNA and protein expression of inducible nitric oxide synthase (iNOS), arginase-1 (Arg-1) and tumor necrosis factor-α (TNF-α). Results Compared with the model group, the BBB score increased (P<0.05), the expression of iNOS and TNF-α de-creased, and the expression of Arg-1 increased (P<0.05). Conclusion Ultrashort wave therapy may promote macrophage polarization from M1 to M2, inhibit inflammatory re-sponse and promote recovery after spinal cord injury in rats.