AA肉鸡免疫禽流感油乳剂灭活疫苗细胞免疫应答的变化

摘要

为了解肉鸡生长期内免疫前后的细胞免疫反应,本实验采用CD3/CD4/CD8三色流式细胞术、MTT法和Griess法依次检测了AA肉鸡免疫禽流感灭活疫苗和非免疫状态下外周血、胸腺和脾脏中T淋巴细胞及其亚群的相对含量、外周血中T淋巴细胞对PHA-P的增殖反应能力和诱导巨噬细胞NO分泌量的变化.结果表明.非免疫状态下,AA肉鸡外周血CD4+、CD8+、CD3+T淋巴细胞相对百分含量在7日龄时均为最低,脾脏中CD4+T淋巴细胞相对百分含量在整个试验期一直呈下降趋势,免疫后外周血、脾脏和胸腺中CD4+、CD8+、CD3+T淋巴细胞相对百分含量均明显高于非免疫组,且脾脏CD4+ T淋巴细胞呈阶段性上升状态;各时期淋巴细胞刺激指数(SI)值和巨噬细胞NO分泌量检测结果表明,免疫组均高于同期对照组(p<0.05).研究结果还表明:肉鸡在7日龄时细胞免疫水平最低,灭活疫苗可以激发肉鸡产生较强的细胞免疫应答.%To investigate cellular immune response in AA broilers, the T lymphocytes were isolated from periphery blood,thymus and spleen. The proliferative ability of T lymphocyte to PHA-P and the changes of NO secretory from induced macrophages in periphery blood from immunized and unvaccinated chickens with inactivated avian influenza virus vaccine were detected by CD3/CD4/CD8 triple labelling for flow cytometric analysis, MTT and Griess assays, respectively. The results show that the percent of CD4+, CD8+ and CD3+ T lymphocyte subpopulations in periphery blood keep the lowest level at 7-day old AA broilers, and the percent of CD4+ T lymphocytes in spleen constantly decreased in the control group during experiment. While the percent of CD4+, CD8+ and CD3+ T lymphocyte subpopulations in periphery blood, spleen and thymus of immunized groups were h igher than that of the control group, and the percent of CD4+ T lymphocytes in spleen consistently increased afer vaccination.Test result of SI-value and NO secretory of macrophages in the different stage displayed that immunized groups were higher than the control groups (p＜0.05). The result also suggests that cellular immunity stay the lowest level at 7-day old and inactivated vaccine could induce strong cellular immune response in AA broilers