猪瘟病毒E2蛋白与猪RACK1相互作用的鉴定

摘要

为筛选和鉴定与猪瘟病毒(CSFV) E2蛋白相互作用的猪宿主蛋白,我们采用酵母双杂交方法筛选猪肺泡巨噬细胞表达文库得到与CSFV E2蛋白相互作用的宿主细胞RACK1蛋白,经共转化试验和GST pull-down 试验进一步证实两者可以特异性结合,并且共聚焦试验表明两者共定位于细胞的细胞浆.本研究显示E2蛋白与宿主细胞RACK1蛋白存在相互作用,RACK1在CSFV感染过程中所发挥的功能有待进一步研究.%To screen and identify the porcine proteins interacted with classical swine fever virus (CSFV) E2 protein, a potential interacting partner of RACK1 was screened with CSFV E2 as bait protein by yeast two-hybrid from porcine alveolar macrophage expression library. The interaction of the two proteins was further confirmed by co-transformation, GST pull-down and laser confocal assays. These results proved that CSFV E2 interacted with RACK1.