山东省规模化猪场PRRSV NSP2变异株的感染情况调查

摘要

To investigate the porcine reproductive and respiratory syndrome virus (PRRSV) with NSP2 mutants,the NSP2 genes were amplified and sequenced from 830 serum samples of clinical healthy pigs collected from twenty scale swine farms in Shandong province during 2010 to 2011,which were immunized with imported and domestic attenuated vaccines respectively.The results showed that the NSP2 mutant PRRSVs were commonly detected on those clinical healthy scale piggery.However,the positive rates of mutant PRRSVs were the highest in the unimmunized swine herds and the lowest in the swine herds immunized with domestic attenuated vaccine.The NSP2 mutant wild virus and vaccine virus were also detected in the same swine herd.%为了解山东省规模化猪场猪繁殖与呼吸障碍综合征病毒(PRRSV) NSP2变异株的感染情况,2010～2011年本研究从山东省未免猪繁殖与呼吸障碍综合征(PRRS)疫苗猪场、免疫进口PRRS弱毒苗猪场和免疫国产PRRS弱毒苗猪场选择20个临床表观健康的规模化猪场,采集830份血清,利用RT-PCR方法对其进行PRRSV NSP2变异株病原学检测.结果表明山东省规模化猪场普遍存在PRRSV NSP2变异株感染,而且临床表观健康的未免疫PRRS疫苗猪场、免疫进口PRRS弱毒苗猪场及免疫国产PRRS弱毒苗猪场中PRRSV NSP2变异株的检出率由高到低,同一猪群中存在PRRSV NSP2变异株野毒和疫苗毒.