影响羊水细胞培养成功率因素的分析

摘要

Objective To Analysis the factors affecting the success rate of amniotic fluid cell culture and to investigate the methods to improve the success rate.Method By the time of improving the method, amniotic fluid cell culture results were divided into two groups.We compared the differences of the two groups of amniotic fluid cells culture method and the success rate.Results In the first group of 515 cases of amniotic fluid,32 cases of amniotic fluid cell culture failed,the failure rate is 6.21 %.In the improved group of 6736 cases of the amniotic fluid,only 18 cases failed,the failure rate is 0.27%.Conclusions Timely and correctly handle the amniotic fluid specimens and control each link effect the success of the cell culture,can improve the success rate of amniotic fluid cell culture.%目的：分析影响羊水细胞培养成功率的因素并探索提高成功率的方法。方法以方法改进时间为界将羊水细胞培养结果分为两组，对比两组羊水细胞培养方法及培养成功率的差异。结果改进前组的515例羊水病例中失败32例，失败率为6．21％。改进后组的6736例羊水病例中仅有18例失败，失败率为0．27％。结论及时、正确处理羊水标本以及控制影响细胞培养成功的各个环节，可提高羊水细胞培养的成功率。