改进超硬组织切片技术用于钛种植体骨界面研究

摘要

目的：研究改进的超硬组织切片技术应用于钛种植体骨界面研究中的关键步骤及需注意的问题。方法本研究于2012年8-11月在山西医科大学动物实验中心完成。选用6只成年雄性Beagle犬，在上颌牙槽骨分别植入6枚种植体后，锯下带有种植体的牙槽骨，采用改进超硬组织切片技术制作切片并进行染色观察。结果60～80μm厚的切片上通过甲苯胺蓝染色可以观察到骨组织的微细结构、种植体与骨组织的结合情况以及胶原纤维和类骨质。结论改进的超硬组织切片技术适合种植体与骨组织结合情况研究。%Objective To study the key steps and matters needing attention in the application of improved technique of super-rigidity slices to research of titanium implant-bone interface. Methods The specimens of implant-bone were collected,super-rigidity slices made by Leica 1600(German)were examined by staining. Results The 60~80μm sec-tions could be obtained with the methods of this study. The histological characteristics of implant-bone interface are ob-served easily. Conclusion The improved techniques in super-rigidity slices provided a good method for the research of implant-bone interface.