微型染色体维持蛋白7在宫颈癌早期诊断和预防中的价值

摘要

目的 探讨微型染色体维持蛋白7 (MCM7)在宫颈癌早期诊断和预防中的意义.方法 2008年1月至2009年10月在皖南医学院附属弋矶山医院就诊的宫颈上皮内瘤变(CIN)1、2、3和宫颈癌患者各40例,慢性宫颈炎20例为对照.采用免疫组化法测定MCM7、细胞周期蛋白E(Cyclin E)、Ki-67在各组中的表达,同时检测人乳头瘤病毒(HPV) DNA分型.结果 MCM7在各组中的阳性表达率均为100％,各组间比较,差异有统计学意义(P＜0.001).宫颈癌与CIN3比较,差异无统计学意义(P＞0.05)；与其他组比较,差异有统计学意义(P均＜0.001).在各组中,高危型HPV阳性率均≤92.5％.在宫颈癌和CIN3中,MCM7的表达水平高于Ki-67和Cyclin E,差异具有显著性(P均＜0.001).结论 MCM7可能是早期诊断和预防宫颈癌的理想标志物.%Objective to investigate significance of minichromosome maintenance protein (MCM) 7 in early diagnosis and prevention of cervical cancer. Methods The expression of MCM7, cyclin E and Ki-67 protein in 40 cases of cervical cancer,40 cases of cervical intraepithelial neoplasia ( CIN) 1, 40 cases of CIN2 and 40 cases of CIN3 were detected by immunohistochemical technique between January of 2008 and October of 2009 in Yijishan Hospital affiliated to Wannan Medical College. HPV DNA typings were simultaneously detected. Results The positive rates of MCM7 were all 100% in all groups. There were significant differences in all groups (P <0. 001). There were no significant differences between cervical cancer and CIN3( P >0.05) ,but significant differences were found between cervical cancer and the other groups (all P < 0. 001 ). In all groups,the positive rates of high-risk human papillomavirus did not exceed 92. 5%. The levels of MCM7 were higher than the one of Ki-67 and CyclinE in cervical cancer and CIN3 ( all P < 0.001). Conclusion MCM7 is an ideal biomarker in early diagnosis and prevention of cervical cancer.