提取方式对香加皮“质量-毒性”综合评价模式的影响

摘要

目的 对香加皮不同组分中4-甲氧基水杨醛的含量及小鼠急性毒性大小进行比较研究,探讨不同提取方式对香加皮质量与毒性之间相关性的影响.方法 分别制备香加皮全组分、水提组分与全组分样品,运用HPLC法与经典的小鼠急毒实验方法,进行香加皮不同组分中主要成分4-甲氧基水杨醛的含量测定及急性毒性比较研究.结果 4-甲氧基水杨醛的含量大小依次为香加皮全组分＞醇提组分＞水提组分；香加皮不同组分对小鼠急性毒性强度为:醇提组分＞水提组分＞全组分,全组分MTD值为16.0 g·kg-1·d-1,水提组分与醇提组分LD50按含生药量计算分别为93.578 g·kg-1·d-1和61.388 g·kg-1·d-1.结论 不同组分中4-甲氧基水杨醛含量与小鼠急性毒性大小均存在显著性差异,全组分4-甲氧基水杨醛含量最高,醇提组分急性毒性最大,4-甲氧基水杨醛是否为香加皮的毒性物质基础之一尚需进一步研究确证.%Objective By comparing the 2-Hydroxy-4-methoxybenzaldehyde's content and the acute toxicity of different extracts in Cortex Periplocae, to explore the effect of different extraction solvents on the quality and toxicity of Cortex Periplocie. Methods By using HPLC and the classic acute toxicity experiment method, to determine the content of 2-Hydroxy-4-methoxybenzaldehyde and compare the acute toxicity of different extracts. Results The 2-Hydroxy-4-methoxybenzaldehyde's content of different composition from the biggest to the smallest is all components, alcohol extraction components and water extraction components. The acute toxicity of different extracts from the biggest to the smallest is alcohol extraction components.water extraction components and all components. The LD^ of water extraction components and alcohol extraction components in Cortex Periplocie are 93.578 g·kg-1·d-1 and the 95% confidence limit is 89.149-98.484 g·kg-1·d-1 which is equal to 1091.8 times of 70kg people's daily dried medicinal herb expenses and 61.388 g·kg-1·d-1 and the 95% confidence limit is 57.466～65.521 g·kg-1·d-1 which is equal to 716.2 times of 70kg people's daily dried medicinal herb expenses. The all-components in Cortex Periplocae is unable to make LD50, while MTD results calculated in accordance with crude drug content is respectively 16.0 g·kg-1· d-1, which is equal to 186.6 times of 70kg people's daily dried medicinal herb expenses. Conclusion There are significant differences on the content of 2- Hydroxy-4-methoxybenzaldehyde and the toxicity. There are the biggest content of 2-Hydroxy 4 methoxybenzaldehyde in the all-components while the toxicity of alcohol extraction components are the biggest. We need do farther study to corroborate whether 2-Hydroxy—4-methoxybenzaldehyde is also one of the toxic substance in Cortex Periplocae.