菌毒清颗粒体外抗病毒的实验研究

摘要

目的：观察菌毒清颗粒的体外抗病毒作用。方法采用细胞病变的 CPE 法观察菌毒清颗粒在体外对呼吸道合胞病毒、腺病毒、柯萨奇病毒的抑制作用，采用实时荧光定量 PCR 法观察菌毒清颗粒对EB 病毒 BcLF 和 BARF1基因表达的抑制作用。结果菌毒清颗粒对呼吸道合胞病毒、腺病毒、柯萨奇病毒所致 CPE 均有明显的抑制作用，对EB 病毒的BcLF 和 BARF1基因表达具有明显的抑制作用。结论菌毒清颗粒能在体外抑制呼吸道病毒、肠道病毒和疱疹科病毒。%Objective To observe the antivirus effects of Junduqing granule in vitro. Methods The cytopathic effect (CPE) method was adopted to study the inhibitory effect of Junduqing granule on respiratory syncytial virus (RSV), adenovirus (AdV) and coxsackie virus (CoxV) in vitro. The quantitative real-time PCR method was used to observe the inhibiting effect of Junduqing granule on EB virus BcLF and BARF1 genetic expression. Results Junduqing granule showed obvious inhibiting effect on the CPE induced by RSV, AdV and CoxV. And it also has obvious inhibiting effect on EB virus BcLF and BARF1 genetic expression. Conclusion Junduqing granule can inhibit respiratory virus, enterovirus and EB virus in vitro.