HPLC法测定麝香追风膏中盐酸麻黄碱含量

摘要

目的 建立高效液相色谱法测定麝香追风膏中盐酸麻黄碱的含量.方法 采用高效液相色谱法,色谱柱为Welch极性乙醚连接苯基柱(5μm,250 mm×4.6 mm),流动相为甲醇-0.092％磷酸溶液(含0.02％二正丁胺和0.04％三乙胺)(1.5:98.5),检测波长210 nm,流速1.0 mL·min-1,进样量10μL.结果 盐酸麻黄碱在1.66× 10-4～0.244 μg范围内呈良好的线性关系,r=0.999 9,平均回收率为101.8％,RSD为1.2％.结论 采用本方法测定麝香追风膏中盐酸麻黄碱含量,准确、方便、专属性强,可用于麝香追风膏的质量控制.%Objective To determine the content of ephedrine hydrochloride in Shexiangzhuifeng ointmen by HPLC.Methods HPLC with Welch polar ether linked phenyl column (5 μm,150 mm×4.6 mm) was employed by using methyl cyanide-0.092％ H3PO4 (0.04％ triethylamine and 0.02％ dibutylamine) (1.5:98.5) as the mobile phase.The flow rate was 1.0 mL · min-1.The detection wavelength was set at 210 nm.The sample size was 10 μL.Results Ephedrine hydrochloride was in good linear relationship within the rage of 1.66× 104～0.244 μg,r=0.999 9.The mean recovery rate was 101.8％ and RSD was 1.2％.Conclusion The HPLC method established here for analyzing the ephedrine hydrochloride in Shexiangzhuifeng ointmen is accurate,convenient and specific,which can be used for the quality control (QC).