柴胡水提物对大鼠解热作用机制研究

摘要

Objective To study the antipyretic effects of water extraction ofBupleurumcomponents for rat fever model and to investigate the antipyretic mechanism.Methods Rat fever model was established via the back injection of 2, 4-dinitrophenol (DNP). Different doses of water extraction ofBupleurum components were given to rats by gavage. The temperature was detected in anus once every 30 minutes, continuously determined for 4 hours, recorded anal temperature and observed the antipyretic effect. At the same time, the levels of IL-1β, IL-6, TNF-ɑ, PGE2 in serum were detected. The content of cyclic adenosine phosphate (cAMP) in hypothalamic and plasma as well as the arginine vasopressin (AVP) levels in brain ventral septal area and plasma. Results The modeling was successful because the rectal temperature of rats increased signiifcantly (P<0.001) after injection of 2, 4-dinitrophenol. The high, medium, low dose of water extraction components ofBupleurum all can reduce the temperature and present a dose dependent relationship, and it was also found that they can decrease the levels of IL-1β, IL-6, TNF-ɑ, PGE2 in serum, the cAMP in hypothalamic and the AVP in brain ventral septal area. However, the content of AVP in plasma was increased, but there was no signiifcant effect on plasma cAMP level.Conclusion Water extraction ofBupleurum components has the antipyretic effect to the rat fever model. The mechanism of antipyretic effect mainly relates to reducing the serum inlfammatory mediators of IL-1β, IL-6, TNF-ɑ, PGE2 in serum and regulating the synthesis and secretion of cAMP and AVP in hypothalamus.%目的：研究柴胡水提物对发热模型大鼠的解热作用并探讨其解热机制。方法采用背部皮下注射2,4-二硝基苯酚，制备大鼠发热模型，灌胃不同剂量柴胡水提物，每30 min测体温1次，连续测定4 h，记录肛温，观察其解热作用；检测血清中白介素-1β（IL-1β）、白介素-6（IL-6）、内皮素（TNF-ɑ）、前列腺素E2（PGE2）水平，下丘脑和血浆中环磷酸腺苷（cAMP）含量以及脑腹中隔区和血浆中精氨酸加压素(AVP)含量。结果注射2,4-二硝基苯酚后各组大鼠肛温显著升高（P<0.001），说明造模成功；柴胡水提物高、中、低剂量组均能降低2,4-二硝基苯酚致热大鼠体温，并呈现一定的剂量依赖关系；检测血中指标发现柴胡水提各剂量组可降低致热大鼠血中IL-1β、IL-6、TNF-ɑ、PGE2、下丘脑中cAMP及脑腹中隔区AVP含量，升高血浆AVP含量，但对血浆cAMP含量无显著影响。结论柴胡水提物对2,4-二硝基苯酚所致大鼠发热模型有一定的解热作用，其作用机制主要与降低血中炎症介质IL-1β、IL-6、TNF-ɑ、PGE2含量和调节下丘脑cAMP及AVP的合成分泌有关。