目的 研究柴胡复方水提物对大鼠亚急性胃溃疡的治疗作用。 方法 大鼠胃浆膜层注射乙酸复制亚急性胃溃疡模型，然后进行实验分组，分别灌胃配给高、低剂量柴胡复方水提物进行治疗，同时以灌注生理盐水组和胃溃疡药Stillen治疗组为对照，通过比较各组间溃疡愈合率、血清中一氧化氮（NO）、超氧化物歧化酶（SOD）活性水平以及观察胃组织形态学的改变，探讨该复方中药对实验性亚急性胃溃疡损伤的保护作用。 结果 阳性药物Stillen组（100 mg/kg）、柴胡复方水提物低剂量（100 mg/kg）和高剂量组（200mg/kg），溃疡面积明显低于溃疡模型组（P<0.01），溃疡愈合率分别为93.5%、40%和92.9%，血清SOD较模型组显著升高（P<0.01）；与模型组比较，阳性药物和柴胡复方水提物低剂量组血清NO增加（P<0.01）；胃组织病理形态学观察，模型组胃黏膜脱落坏死，大量炎性细胞浸润，阳性药物组和柴胡复方高低剂量组胃组织病变有明显改善。 结论 柴胡复方水提物对大鼠亚急性胃溃疡模型有明显的治疗作用。%Objective To study effects of radix bupleuri compound water extraction on prevention in rats with subacute gastric ulcer. Methods The model of subacute gastric ulcer was established by acetic acid to observe effects of radix bupleuri compound water extraction on therapeutical effect in rats with subacute gastric ulcer. The ulcer areas and healing ratio of ulcer were measured. The slices of gastric tissues were observed after HE staining. Nitric oxide (NO) and superoxide dismutase (SOD) in serum were measured by nitric oxide reductase assay and xanthine oxidase assay. Results In Stillen group (100 mg/kg), low dose of radix bupleuri compound water extraction group (100 mg/kg) and high dose group (200 mg/kg), the ulcer areas were decreased compared with those in model group (P<0.01). The ulcer inhibition ratios were 93.5%、40%and 92.9%, and the activities of SOD in serum were increased in drug-administrated groups (P<0.01). The content of NO of serum in radix bupleuri compound water extraction groups were increased compared with those in model group (P<0.05). In model group, deciduous and necrotic gastric mucosas with inflammatory cells infiltration were observed. Morphological changes were improved in drugs-administrated groups. Conclusion Radix bupleuri compound water extraction can be a therapeutical effect on the rats with subacute gastric ulcer.
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