目的 观察苜蓿总皂苷长期给药对Beagle犬的毒性反应.方法 Beagle犬分为苜蓿总皂苷高、中、低剂量组(分别为3.2、1.6、0.8 g·kg-1)和空白对照组,每组8只.连续灌胃给药180 d,观察Beagle犬的一般状态,于末次给药后和停药30 d后分别取部分动物,检测血液学指标、血液生化学指标及病理组织学改变.结果 与空白对照组比较,各给药组对Beagle犬的生长、行为等无显著影响;血液学和血液生化学分析中,苜蓿总皂苷高剂量组白细胞计数增多、丙氨酸氨基转移酶含量降低,中剂量组总蛋白水平升高,低剂量组肌酐水平升高,原因可能与个别动物的应激反应或个体差异有关;血生化其他各项指标均在正常值范围内,给药组与空白对照组比较差异均无统计学意义;病理学检查未见与受试药物相关的变化.恢复期未发生药物引起的迟发性毒性反应.结论 苜蓿总皂苷(0.8～3.2 g·kg-1)对Beagle犬经口长期给药,未见明显毒性反应.%Objective To investigate the long-term toxicity of oral treatment of total saponins from Medicago sativa L.(TSM) on Beagle dogs.Methods Beagle dogs were divided into 4 groups at random (n=8),namely normal control,TSM high,medium and low dose groups (3.2,1.6 and 0.8 g· kg-1,respectively).TSM was orally administered for 180 days.The body weight,behavior and food intake of animals were observed during the administration.The hematology and blood biochemistry analysis were carried out,and organ histopathological changes were observed under the microscope at the end of the administration and 30 days after the administration,respectively.Results The outer appearance,behavior and body weights in dogs of TSM groups showed no significant differences compared to those of normal control group.In hematology and blood biochemistry analysis,white blood cell count was increased while aminopherase activity was decreased in the blood of TSM high dose group.The levels of total protein and creatinine were significantly increased in TSM medium and low dose group,respectively.This may attribute to the stress response or individual difference of some Beagle dogs.In addition,no changes related to TSM among other indices were observed.TSM showed no apparent influence in organs of doggs in histopathological observation.In addition,no delayed toxicity reaction was showed 30 days after the administration.Conclusion TSM (0.8 to 3.2 g·kg-1) shows no obvious toxicity in Beagle dogs for long-term administration.
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