扇贝裙边糖胺聚糖对大鼠血管平滑肌细胞增殖的影响

摘要

目的研究扇贝(Placopecta magellanicus)裙边中提取的糖胺聚糖(SS-GAG)是否如肝素一样抑制血管平滑肌细胞(VSMC)的增殖.方法采用胎牛血清和碱性成纤维细胞生长因子(bFGF)所诱导的大鼠胸、腹主动脉VSMC增殖模型,通过细胞计数、结晶紫染色及MTT比色法观察SS-GAG对VSMC增殖的影响,并运用免疫组织化学技术和计算机图像分析系统检测SS-GAG对VSMC的增殖细胞核抗原(PCNA)和血小板衍化生长因子(PDGF)表达的影响.结果 SS-GAG 50,100和200 mg·L-1对20% 胎牛血清和50 μg·L-1 bFGF所诱导的VSMC增殖均有明显抑制作用,并能抑制VSMC增殖时PCNA 和PDGF的表达.结论 SS-GAG能抑制VSMC的增殖,该作用可能是通过抑制PDGF和PCNA的表达而实现的.%AIM To investigate if scallop (Placopecta magellanicus) skirt glycosaminoglycan (SS-GAG) inhibits the proliferation of vascular smooth muscle cell (VSMC) as heparin does so and to clarify its mechanism. METHODS The inhibitory effects of SS-GAG on the proliferation of rat thoracic aorta and abdominal aorta VSMC induced by fetal bovine serum (FBS) or basic fibroblast growth factor (bFGF) were determined by cell counting, crystal violet staining and MTT colorimetry. The effects of SS-GAG on the expression of proliferating cell nuclear antigen (PCNA) and platelet-derived growth factor (PDGF) in VSMC proliferation induced by bFGF were evaluated by immunohistochemical technique (LSAB method) and computer image analysis system. RESULTSSS-GAG exerted antagonistic effects on VSMC proliferation induced by 20% FBS and 50 μg·L-1 bFGF at concentrations ranging from 50 mg·L-1 to 200 mg·L-1 and repressed the increasing expression of PCNA and PDGF. CONCLUSION SS-GAG significantly inhibits the proliferation of VSMC, which may be carried out through repression of PDGF and PCNA expression.