小儿恶性肿瘤突变型P_(53)蛋白的检测及临床意义

摘要

采用免疫组织化学方法检测小儿常见恶性肿瘤突变型P_(53)蛋白的表达情况并探讨其临床意义.甩DO-1P_(53)单克隆抗体,LSAB法测定45例小儿恶性肿瘤石蜡切片的P_(53)表达.19例神经母细胞瘤3例阳性,均为Ⅳ期患儿,其中1例阳性为睾丸转移瘤而原发瘤阴性;16例肾母细胞瘤4例阳性,其中3例预后不良型均为阳性;10例恶性淋巴瘤5例阳性,均为Ⅰ、Ⅳ期患儿.结果表明突变型P_(53)蛋白的表达与三种肿瘤的临床分期、组织学类型有关,可能为三种肿瘤转移、复发的原因,为小儿恶性肿瘤预后不良的一个重要指标.%Objective:To detect the expression of mutant P53 in Children's malignant tumors by immunohistochemical method and to discuss its clinical significance.Method:45 polysine-coated slides were stained with DO-1 P_(53) monoclonal antibodies by the method of LSAB.Results:There were 3 cases(stage Ⅳ)of positive mutant P_(53) in 19 neuroblastomas.Of them,one case of testicular metastatic tumor was positive,but the primary tumor was negative.Four cases(UF type in 3)of the 16 nephroblastomas were positive.Five of the 10 malignant lymphomas were positive.All of them belonged to stage Ⅲ orⅣ.Conclusion:The expression of mutant P_(53) is related to clinical stage and histologic type of the above three tumors.It is probably a cause of tumor metastasis and relapse.The mutant P_(53) being positive appears to be an important prognostic factor of some tumors in children.