肉碱对高脂饲料喂养的半饥饿大鼠脂肪代谢的改善作用

摘要

AIM: To investigate the effects of exogenous L - carnitine on lipid metabolism in semi - starved rats fed on high fat diet. METHODS: The semi - starved rats were restricted half in calorie intake on high fat diet for 2 week. L - carnitine was supplied at dose of 250 mg/kg· bw. The changes of plasma carnitine concentration, urinary excretion of ketone body, serum lipase activity, muscle carnitine palmitoyl transferase activity, triglyceride secretion and clearance rate were measured. RESULTS: The results showed that the concentration of plasma free camitine increased significantly in carnitine supplemented rats compared to normal and semi - starved rats. The activities of muscle carnitine palmitoyhransferase and serum lipase were significantly enhanced in carnitine supplemented rats. The triglyceride secretion rate (TGSR) was also improved remarkably by carnitine supplementation. Meanwhile, the urinary excretion of ketone body was reduced significantly in carnitine supplemented rats compared to semi - starved rats. CONCLUSION: It is concluded that carnitine supplementation can significantly increase the plasma concentration of free carnitine and accelerate the lipid metabolism in semi - starved rats fed on high fat diet.%目的：探讨补充外源性肉碱对高脂饲料喂养的半饥饿大鼠脂肪代谢的影响。方法：用限食的方法造成半饥饿大鼠模型，在给予高脂饲料基础上，观察补充肉碱的半饥饿动物2周后血浆肉碱浓度、附睾脂肪垫重量、尿酮体排出、血浆脂肪酶活性、肌肉肉碱棕榈酰基转移酶活性以及甘油三酯分泌及清除速率等指标的变化。结果：补充肉碱的动物血浆游离肉碱浓度显著高于正常对照组和半饥饿对照组；尿酮体排出显著低于半饥饿对照组；血浆脂肪酶活性和肌肉肉碱棕榈酰基转移酶活性显著高于正常对照组和半饥饿对照组；甘油三酯分泌速率也显著高于半饥饿对照组。结论：补充外源性肉碱能够显著提高高脂饲料喂养的半饥饿大鼠血中游离肉碱浓度，加速动物体内脂肪代谢。