AIM: To investigate the possibility of 3T3 fibroblast growth on blood fibrin clot in culture medium with recombinant human basic fibroblast growth factor (rhbFGF). METHOD: Growth of the cells on blood fibrin clot was studied by phase-contrast, scanning and transmission electron microscopy and by Giemsa stain and MTT assay. RESULTS: The optimal concentration of rhbFGF for proliferation and survival of the cells was 100 ng/mL. The cells also grew on blood fibrin clot scaffold in the low-serum medium containing 100 ng/mL rhbFGF, and a greater number of the cells survived after 48 hours incubation compared to that after 24 hours. The elongated filopodia appeared to bridge the gaps among the fibroblasts after 24 hours incubation. Further incubation to 72 hours, a greater number of platycytes were found to be joined together by lamellopodia. CONCLUSION: 3T3 fibroblasts could grow and survive on blood fibrin clot in the low-serum medium containing rhbFGF, and a combination of blood fibrin clot and rhbFGF may have over proportional effects on wound healing.%目的:重组碱性成纤维细胞生长因子(rhbFGF)对培养条件下3T3成纤维细胞在血纤维蛋白凝块上生长的可能性研究.方法:采用Giemsa染色和MTT检测法,经过电镜扫描,分段对比观察,研究3T3细胞在血纤维蛋白凝块上的生长情况. 结果:rhbFGF促进细胞生长并维持细胞存活的最佳浓度是100 ng/mL,在含有100 ng/mL的低血清培养基中细胞可在血纤维蛋白凝块上生长.经48h培养以后仍有大量的细胞存活. 结论:3T3成纤维细胞可在含有rhbFGF的低血清培养基上生长并存活,rhbFGF与血纤维蛋白凝块共存可以促进创伤愈合.
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