PPARδ激动剂GW501516抑制ox-LDL或高糖诱导的人脐静脉内皮细胞凋亡

摘要

AIM:To investigate the inhibitory effect of peroxisome proliferator - activated receptor 8 ( PPAR8) agonist GW501516 on the apoptosis induced by oxidized low-density lipoprotein(ox-LDL) or high concentration of glucose in human umbilical vein endothelial cells ( HUVECs). METHODS; Cell apoptosis was induced by ox - LDL or high concentration of glucose in HUVECs and was examined by flow cytometry. The HUVECs were treated with GW501516 at different concentrations. The viability of HUVECs was analyzed by MTT assay. RESULTS; The apoptosis rate of HUVECs treated with ox - LDL was 21. 3% , while those of HUVECs treated with ox - LDL combined with low, medium and high concentrations of GW501516 were 17.47% , 9. 72% and 3.94% , respectively. The apoptosis rate of HUVECs treated with glucose was 22. 60% , while those of HUVECs treated with glucose combined with different concentrations of GW501516 were 20. 23% , 17. 01% and 9. 38% , respectively. The results indicated that ox - LDL or glucose induced apoptosis of HUVECs and GW501516 decreased the apoptotic rates induced by ox - LDL or glucose in a dose - dependent manner. The results of MTT assay showed that glucose or ox - LDL decreased the viability of HUVECs and GW501516 attenuated the effect of glucose or ox - LDL on HUVECs. CONCLUSION; GW501516 inhibits the apoptotic effects of ox - LDL and glucose on HUVECs and increases the viability of the cells.%目的:探讨过氧化物酶体增殖物激活受体δ(PPARδ)激动剂GW501516对氧化低密度脂蛋白(ox-LDL)和高糖诱导的人脐静脉内皮细胞(HUVECs)凋亡的抑制作用.方法:ox-LDL或高糖诱发HUVECs凋亡,给予不同浓度GW501516处理后,用流式细胞术检测细胞凋亡率的变化.采用MTT法检测ox-LDL或高糖对HUVECs生长活性的影响,用不同浓度GW501516干预后观察细胞生长活性的变化.结果:ox-LDL诱导的细胞凋亡率是21.30%,不同浓度GW501516处理组凋亡率分别为17.47%、9.72%和3.94%,高糖诱导的细胞凋亡率为22.65%,不同浓度GW501516处理组凋亡率分别为20.23%、17.01%和9.38%,结果显示GW501516可抑制ox-LDL或高糖诱导的细胞凋亡,且呈剂量依赖性;MTT结果显示,GW501516可使ox-LDL或高糖对HUVECs活性的抑制作用减弱.结论:GW501516可抑制ox-LDL或高糖诱导的HUVECs凋亡;并减弱ox-LDL或高糖对HUVECs活性的抑制作用.