口虾蛄提取物对人鼻咽癌细胞P53、COX-2和VEGF表达的影响

摘要

AIM : To study the inhibitory effect of the extract of Oratosquilla( EOS ) on the expression of P53 ,cyclooxygenase - 2( COX - 2 ) and vascular endothelial growth factor( VEGF ) in nasopharyngeal carcinoma cell line CNE 2Z.METHODS : CNE - 2Z cells were treated with different concentrations of EOS for 24 h.The methods of RT - PCR and immunocytochemistry were used to detect the expression of COX - 2 and VEGF at mRNA and protein levels, respectively.The protein expression of P53 was determined by Western blotting.RESULTS : After treated with EOS, the protein expression of P53 in CNE - 2Z cells was decreased( P ＜ 0.01 ), and the expression of COX -2 and VEGF at mRNA and protein levels was also significantly decreased in a dose - dependent manner( P ＜ 0.01 ).The expression of COX - 2 was positively correlated with that of VEGF( P ＜ 0.05 ), and a positive correlation hetween the expression of COX - 2 and P53 protein( P ＜ 0.05 ) was also observed.CONCLUSION : EOS may play its antitumor effect by inhibiting the expression of P53 , COX - 2 and VEGF in nasopharyngeal carc:inoma cell line CNE - 2Z.%目的:研究口虾蛄提取物(EOS)对人低分化鼻咽癌细胞系(CNE-2Z)中P53、环氧化酶2(COX-2)和血管内皮生长因子(VEGF)表达的影响及意义.方法:不同浓度EOS(0 mg/L、125 mg/L、250 mg/L和500 mg/L)作用CNE-2Z细胞24 h后,Western blotting检测P53蛋白表达,免疫细胞化学法及RT-PCR法分别检测COX-2 、VEGF蛋白和mRNA的表达.结果:CNE-2Z细胞中P53蛋白、COX-2和VEGF蛋白和mRNA表达均随EOS作用浓度增高而逐渐降低,呈量效关系(P＜0.01);且COX-2 和VEGF表达量呈正相关(P＜0.05),COX-2 和P53表达量亦呈正相关(P＜0.05).结论:EOS可能通过抑制CNE-2Z细胞中P53蛋白的表达,干扰COX-2和VEGF的表达而发挥其抗肿瘤作用的.