HA/ZrO2复合材料颗粒的体外基因毒性的初步研究

摘要

AIM: To evaluate the genotoxicity of the hydroxyapatite/ZrO2( HA/ZrO2 ) composite particles by using the in vitro micronucleus test ( MNT ).METHODS : The HA/ZrO2 composite particles prepared by sintering at high temperature and pressure using the powder of HA and ZrO2 with different proportions were compared with the pure HA particle and pure ZrO2 particle.The suspension of the composite particles was made.The rahbit mesenchymal stem cells were isolated and cultured.The promotive effect of the composite particles on the proliferation of the rabbit mesenchymal stem cells was detected by MTT method.The genotoxicity of the composite particles to the rabbit mesenchymal stem cells was measured hy MNT method.RESULTS : The MTT test showed that both of the pure HA particle and the composite particles containing HA promoted the proliferation of rabbit mesenchymal stem cells.However, no promotive effect of the pure ZrO2particle on the proliferation of rabbit mesenchymal stem cells was observed.The MNT test showed no significant difference between HA group and negative control group ( P ＞ 0.05 ), and significant difference between HA group and positive control group ( P ＜0.05 ).The difference between Zro2 group and negative control group was significant ( P ＜ 0.01 ), and the difference between ZrO2 group and positive control group was insignificant ( P ＞ 0.05 ).CONCLUSION : The increase in genotoxicity of HA/ZrO2 composite particle is dependent on the proportion of ZrO2 and the concentration of the composite.The 30％ wt HA/70％ wt ZrO2 composite at the concentration of 200 mg/L shows significant genotoxicity.%目的:采用体外微核法评价HA/ZrO2复合材料颗粒的基因毒性.方法:按不同比例将羟基磷灰石与二氧化锆粉体混合在高温高压下烧结制备HA/ZrO2复合材料颗粒,纯HA和纯ZrO2颗粒作为对照.制备复合材料颗粒的悬液,分离和培养兔间充质干细胞.MTT法检测复合材料颗粒悬液对兔间充质干细胞增殖促进作用,体外微核试验(MNT) 检测复合材料颗粒悬液对兔间充质干细胞的基因毒性.结果:MTT实验显示:纯HA和含HA的复合材料颗粒均对间充质干细胞增殖有一定促进作用,而纯ZrO2颗粒对间充质干细胞增殖没有促进作用,差异显著(P<0.05).MNT实验显示:HA组与阴性对照组相比差异不显著(P>0.05),与阳性对照组比差异显著(P<0.05); ZrO2组与阴性对照组比差异显著(P<0.01),与阳性对照组比差异不显著(P>0.05).结论:HA/ZrO2复合材料颗粒的基因毒性随着ZrO2比例和复合材料浓度的增加而增加,30%wtHA/70%wtZrO2在200 mg/L时具有显著基因毒性(P<0.01).