赫赛汀诱导子宫内膜癌 Ishikawa 细胞凋亡并增强其对化疗的敏感性

摘要

AIM:To investigate the role of Herceptin in the apoptosis and drug sensitivity of endometrial canc -er Ishikawa cells .METHODS: The IC50 values of Herceptin , adriamycin ( ADR ) , cisplatin ( DDP ) and paclitaxel ( PTX) for Ishikawa cells were detected by MTT method .Ishikawa cells were treated with single drug and combined chemo-therapy for 24 h, the cell cycle and the apoptosis ratio were determined by flow cytometry .RESULTS:The IC50 values of Herceptin, ADR, DDP and PTX were 57.12 mg/L, 0.572μmol/L, 67.4μmol/L and 719.5 nmol/L, respectively.Her-ceptin significantly enhanced the cytotoxicity of the chemotherapeutic drugs , and increased apoptosis ratio statistically . CONCLUSION:Herceptin enhances the apoptosis-inducing ability of the chemotherapeutic drugs and improves the che-motherapeutic sensitivity in Ishikawa cells .%目的：研究赫赛汀单独或联合阿霉素（adriamycin，ADR）、顺铂（cisplatin，DDP）及紫杉醇（paclitax－el，PTX）对子宫内膜癌细胞凋亡和化疗敏感性的影响，为临床应用赫赛汀治疗子宫内膜癌提供理论依据。方法：MTT法检测赫赛汀、ADR、DDP及PTX处理子宫内膜癌Ishikawa细胞的IC50。进一步应用1／2 IC50量的赫赛汀与各1／2 IC50量的ADR、DDP及PTX药物联合，流式细胞术检测细胞周期与凋亡变化。结果：赫赛汀抑制子宫内膜癌Ishikawa细胞生长，引起细胞G1期阻滞，诱导细胞凋亡。子宫内膜癌Ishikawa细胞应用赫赛汀、ADR、DDP及PTX的IC50分别为57．12 mg／L、0．572μmol／L、67．4μmol／L和719．5 nmol／L，赫赛汀联合化疗后显著提高各组化疗药的杀伤效果，诱导细胞凋亡，与单纯化疗组相比差异有统计学意义（P＜0．05）。结论：赫赛汀诱导子宫内膜癌细胞凋亡，并提高子宫内膜癌细胞株对化疗的敏感性。