pEZRThr567在肺鳞癌中的表达及临床病理学意义

摘要

目的：探讨第567位苏氨酸磷酸化的埃兹蛋白（pEZRThr567）在肺鳞癌、癌旁及正常肺组织中的表达及其临床病理学意义。方法：用免疫组化法检测pEZRThr567在肺癌、癌旁及正常肺组织中的表达，并分析其异常表达与临床病理学特点之间的相关性；用免疫荧光染色法检测pEZRThr567在肺鳞癌细胞EBC-1中的定位情况。结果：pEZRThr567蛋白在肺鳞癌组织中的表达明显高于癌旁组织和正常肺组织（P＜0．01）；pEZRThr567蛋白主要表达于细胞膜，且其表达与肿瘤分化程度、临床分期和有无淋巴结转移密切相关（P＜0．05）。结论： pEZRThr567蛋白可作为肺鳞癌恶性进展的有效分子指标。%AIM:To investigate the clinicopathological significance of the protein expression of phosphorylated ezrin at threonine 567 ( pEZRThr567 ) in lung squamous cell carcinoma, adjacent tissues and normal tissues.METHODS:pEZRThr567 protein was detected in lung squamous carcinoma, adjacent and normal tissues by the method of immunohisto-chemistry.The correlation of pEZRThr567 expression with clinicopathological parameters of lung squamous carcinomas was al-so analyzed.The localization of pEZRThr567 was detected by immunofluorescence staining in lung squamous cell line EBC-1. RESULTS:The protein expression of pEZRThr567 in lung squamous carcinoma was significantly higher than that in the adja-cent and normal lung tissues (P<0.01).pEZRThr567 mainly localized on the cell membrane, and its over-expression signi-ficantly correlated with the differentiation, clinical stage and lymph node metastasis in lung squamous carcinoma.CON-CLUSION:pEZRThr567 may be an effective biomarker for prediction of malignant potential and poor prognosis of lung cancer.