目的:探讨补阳还五汤(BYHWD)促进内皮祖细胞(EPCs)修复损伤血管内皮的作用及相关机制.方法:以补阳还五汤灌胃及EPCs尾静脉输注作用于内皮损伤的模型大鼠,从血管内皮的形态、功能和EPCs归巢等方面评价内皮损伤修复情况;从血管内环境改善和基质细胞衍生因子-1(SDF-1)及其受体趋化因子受体-4相关蛋白表达了解该方促进EPCs修复受损血管的机制.结果:与单用EPCs组和单用BYHWD组比较,BYHWD联合EPCs组内膜厚度明显减少,甘油三酯、总胆固醇及血钙含量降低,高密度脂蛋白含量升高;此外,BYHWD联合EPCs组血管内皮型一氧化氮合酶(eNOS)及血管SDF-1蛋白表达均显著增加.结论:补阳还五汤能促进EPCs修复损伤的血管内皮,其作用机制可能与调控内环境以及促进EPCs的归巢有关.%AIM:To investigate the role of Buyanghuanwu decoction(BYHWD) in promoting endothelial pro-genitor cells(EPCs)-induced recovery of damaged vascular endothelium. METHODS:The endothelial damaged rats were lavaged with BYHWD and injected with EPCs through vena caudalis. The repaired situation of damaged endothelium was observed. RESULTS:Compared with EPCs group and BYHWD group,the endothelial thickness was reduced, the levels of calcium,triglycerides and total cholesterol were decreased,but the high density lipoprotein levels were increased. In ad-dition,the protein expression of vascular endothelial nitric oxide synthase and vascular stromal cell-drived factor-1 was sig-nificantly increased,but the expression of CXC chemokine receptor-4 was significantly reduced in BYHWD+EPC group. CONCLUSION:BYHWD promotes EPCs repairing damaged endothelium,the mechanism may be related to improve the internal environment and promotes the EPCs homing.
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