MTA2在舌癌组织中的表达及其意义

摘要

Objective To observe the expressions of mRNA and protein of metastasis-associated protein 2 (MTA2) in tongue carcinoma and explore its clinical significance.Methods Protein expression of MTA2 in 95 specimens of tongue carcinoma and 25 of para-tumor normal mucosa epithelial tissue was detected by immun ohistochemical method.The expressions of MTA2 mRNA and MTA2 protein in 20 specimens of tongue carcinoma and 20 of para-tumor normal tissue were measured by real-time polymerase chain reaction (RT-PCR) and Western Blot respectively.The relationships between the expressions and patients' gender,age and the degree of differentiation of carcinoma tissue were analyzed.Results (①) The expressions MTA2 mRNA and MTA2 protein in the tongue carcinoma tissues were significantly increased compared with the para-tumor normal tissues (both P ＜ 0.05).②) There were no significant relationships between the expressions and patients' gender and age (all P ＞ 0.05) while the expressions were negatively correlated with the differentiation degree of carcinoma (both P ＜ 0.05).Conclusions MTA2 expression in tongue carcinoma tissue is higher than that in the para-tumor normal tissue.MTA2 expression is negatively correlated with the differentiation degree of carcinoma.%目的 比较舌癌患者和正常人中转移相关蛋白2(metastasis associated protein 2,MTA2)的mRNA和蛋白表达水平,并探讨其差异的临床意义.方法 选取经病理确诊为舌鳞癌患者95例,同时选取癌旁正常舌黏膜25例;同期另各取20例舌癌组织与20例癌旁正常上皮组织,采用免疫组化方法检测95例确诊为舌癌组织及25例相应癌旁正常舌黏膜上皮组织中MTA2的表达;采用RT-PCR和Western Blot分别检测20例舌癌组织与癌旁正常上皮组织中mRNA和蛋白的表达.分析MTA2在舌癌组织中的表达与患者的性别、年龄及癌组织分化程度的关系.结果 MTA2的mRNA和蛋白在舌癌组织中的表达明显高于癌旁组织(P＜0.05).MTA2在舌癌组织中的表达与患者性别、年龄无显著相关(P＞0.05),而与癌组织的分化程度呈负相关(P＜0.05).结论 MTA2在舌癌组织中的表达高于癌旁组织,MTA2的表达与癌组织的分化程度呈负相关.