抗原处理相关转运体蛋白1 637A/G基因多态性与中国云南省汉族鼻咽癌发病风险关系的研究

摘要

objective To study the relationship between 637A/G gene polymorphisms of the transporter associated with antigen processing 1 gene and nasopharyngeM carcinomas(NPC)and to find out the susceptible genes of NPC in Han population in Yunnan Province.China.Methods Two hundmd and thirtv-three cases with NPC and 296 cases matched cancer-free controls were genotyped for the TAP1 637 A/G polymorphism by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).Epstein-Barr virus infection was detected by PCR.The adiusted edds ratios(OR)and 95%confidence intervals(CI)were calculated by using unconditional logistic regression model.Results Contrast with homozygous TAP1 637 AA.G allele significandy increasing risk of NPC was associated with homozygous 637GG OR=4.26(95%CI were 2.08-8.66.P<0.001)and heterozygous 637 AG OR=1.56(95% CI were 1.12-2.33,P<0.05).The subjects at least having one TAP1 637 G allele had OR of 1.88(95%CI were 1.35-2.82,P<0.001).Furthermore,EBV infection may increase the risk of developing NPC interacting with TAP1 637 A/Gpolymorphism OR=2.76(95%CI were 1.69-4.63,P.<0.001).Condusions The TAP1 637G allele is associated with the NPC in Han population in Yunnan China, EBV pathogenesis in NPC might be facilitated by polymorphisms in the TAP1 proteins.[ Key words ] Nasopharyngeal neoplasms; ATP-binding cassette transporter; Polymorphisms,genetic; Disease susceptibility; Epstein-barr virus infections%目的 研究抗原处理相关转运体蛋白1(transporter associated with antigen processing 1,TAP1)637A/G基因多态性与中国云南汉族鼻咽癌发病风险的关系,寻找鼻咽癌的易感基因.方法 应用聚合酶链反应-限制性片段长度多态性对233例云南籍汉族鼻咽癌患者和296名云南籍汉族正常人对照组进行TAP1 637A/G基因多态性检测,采用PCR检测鼻咽癌患者EB病毒(EB virus,EBV)感染情况.计算各基因型与鼻咽癌发病风险以及与鼻咽癌患者EBV感染的相关性.结果 233例云南鼻咽癌患者中EBV感染率为58.4%,对照组EBV感染率为42.2%,差异有统计学意义(X~2=13.586,P<0.05).比较各基因型在两组中的分布,纯合子突变(GG型)、杂合子突变(AG型)和无突变者(AA型)分布差异均有统计学意义(P值均<0.05).与从基因型相比,携带GG、AG基因型罹患鼻咽癌的风险分别为4.26倍(95%CI为2.08～8.66,P<0.001)和1.56倍(95%CI为1.12～2.33,P<0.05);至少携带637G等位基因罹患鼻咽癌的风险为1.88倍(95%CI为1.35～2.82,P<0.001).TAP1三种基因型频率在EBV阳性组与EBV阴性组间比较,差异有统计学意义(P<0.05);在EBV阳性组中至少携带637G等位基因罹患鼻咽癌的风险为2.76倍(95%CI=1.69～4.63,P<0.001).结论 TAP1 637位点A/G基因多态性与云南汉族鼻咽癌遗传易感相关,TAP1基因多态性干扰人类白细胞抗原I型抗原的呈递可能与EBV感染致云南汉族鼻咽癌患病相关.