肿瘤干细胞标记物USP22蛋白在喉鳞状细胞癌中的表达及预后分析

摘要

Objective To investigate the expression of cancer stem cell marker USP22 in laryngeal squamous cell carcinoma (LSCC) and clinical implications.Methods The expression of USP22 was detected by immunohistochemistry in LSCC tissues of 64 cases and squamous epithelium tissues beside carcinoma of 26 cases (control).The correlation of USP22 expression with various clinicopathologic factors was evaluated with the single factor analysis.Results The expression levels of USP22 in LSCC and control were 57.8％ and 19.2％ (P ＜ 0.05).Clinicopathological analysis showed that USP22 expression level had a relationship with clinical stage,T stage,and lymph node metastasis(P ＜ 0.05),but not with gender,age,smoking and differentiation(P ＞ 0.05).Survival analysis showed that patients with high USP22 expression had significantly poorer outcome compared with patients with low USP22 expression.The survival was related to clinical stage,T stage,and lymph node metastasis,but not with age,sex,and smoking (P ＞ 0.05).Conclusions The expression of USP22 is significantly increased in LSCC,which correlates with the malignant degree,invasion,metastasis and prognosis of LSCC.USP22 may be served as a new candidate molecular marker and therapeutic target of LSCC.%目的 探讨肿瘤干细胞标记物泛素特异性肽酶22(USP22)蛋白在喉鳞状细胞癌(鳞癌)中的表达水平及其与临床分期、分化程度、淋巴转移及预后等临床病理参数的关系.方法 用免疫组化法检测USP22蛋白在64例喉鳞癌组织和26例正常喉黏膜(对照组)组织中的表达,分析其与临床病理学特征的关系.Kaplan-Meier法与Log-rank检验分析生存情况.结果 USP22蛋白在喉鳞癌组和对照组的阳性表达率分别为57.8％和19.2％ (P ＜0.05),其表达分别在不同的临床分期、T分期及淋巴转移之间差异有统计学意义(P值均＜0.05),而在性别、年龄、吸烟、分化程度及分型之间差异无统计学意义(P值均＞0.05);生存分析结果显示喉癌患者USP22蛋白高表达比USP22低表达的预后差;不同分型的5年生存率由高到低的顺序是声门型、声门上型、声门下型.单因素临床分期、T分期、淋巴结是否转移、分化程度对预后的影响有统计学意义(P值均＜0.05),而年龄、性别、吸烟与患者的预后无关(P值均＞0.05).结论 USP22蛋白在喉鳞癌组织中表达明显增高,其高表达与喉鳞癌的恶性程度、侵袭转移及预后有关.USP22蛋白可能是一个新的候选喉癌干细胞标志物和治疗的靶点.