Objective To establish a hyperbilirubinemia model using newborn Wistar rats and evaluate its effect on the auditory system. Method Twenty healthy 7-day-old Wistar rats of either sex were randomly divided into two groups (n= 10). Rats in the treatment group received subcutaneous injection of phenylhydrazine at SO mg/kg for 2 days, while those in the control group received subcutaneous injection of physiological saline. Behavioral changes were observed and morphological changes of spiral ganglions and hair cells examined. Results The animals with induced hemolysis showed signi?cantly increased concentrations of total, direct and indirect bilirubin. Animals in the experiment group exhibited abnormal behavior, including severe movement disorders, lack of instinctive movement, head shaking and tidal breathing. Cochlear hair cells and spiral ganglion cells showed no signs of injury in both group, although neurofilaments were significantly reduced in the experimental group. Conclusion Intraperitoneal injection of phenylhydrazine can be used to build an animal model of newborn hyperbilirubinemia with indicative behaviors. While hair cells and spiral ganglion cells are not affected by the treatment, neurofilaments are significantly reduced .suggesting that hyperbilirubinemia may mainly damage the peripheral auditory system.%目的 建立新生Wistar大鼠高胆红素血症模型,评估高胆红素血症对听觉系统的影响.方法:选择正常新生7天Wistar大鼠20只,随机分为2组,每组10只,实验组连续2天皮下注射盐酸苯肼50mg/kg,对照组皮下注射生理盐水,观察各组实验动物的行为改变及毛细胞和螺旋神经节等结构的形态学变化.结果:实验组较对照组血清总胆红素、直接胆红素、间接胆红素水平均升高.实验组的大鼠行为明显异常,出现严重运动障碍,本能运动缺乏,甚至出现摇头现象和潮式呼吸.各组的耳蜗毛细胞无明显变化,螺旋神经节细胞结构基本正常,实验组较对照组其间穿行神经纤维明显减少.结论:皮下注射盐酸苯肼溶液可以造成高胆红素血症模型；高胆红素血症动物表现为行为异常,耳蜗毛细胞、螺旋神经节无明显损伤,主要损伤其间穿行的神经纤维,提示高胆红素血症可能主要损伤周围听觉系统.
展开▼
