目的：庆大霉素是一种临床上常用的氨基糖苷类抗生素，随着其使用剂量和作用时间的增加，它的耳毒性逐渐明显。本实验在体外用新生C57BL/6J小鼠的耳蜗基底膜构建庆大霉素耳毒性模型观察自噬的发生。方法体外原代培养的耳蜗基底膜应用庆大霉素之后，行透射电镜观察自噬体的结构，免疫荧光、免疫印迹检测自噬相关蛋白LC3-II的变化。结果在庆大霉素的耳毒性模型中，观察到了自噬现象的发生。结论自噬参与了庆大霉素的耳毒性过程，提示我们干预自噬可以影响庆大霉素的耳毒性过程。%Objective Gentamicin is a frequently used aminoglycoside antibiotic. With increasing dose and duration, its ototoxicity side effects will gradually appear. The current study aims to study the phenomenon of autophagy in gentami-cin induced ototoxicity. Methods The cochlea was cultured with gentamicin exposure. Transmission electron microscopy, immunofluorescence and Western blotting were used to observe the expression of autophagy protein LC3 in the explant cul-ture treated with the gentamicin. Results In vitro administration of gentamicin in mouse cochlear cultures induced the for-mation of autophagic vesicles and autophagosomes in hair cells. Conclusion Our results demonstrate that autophagy partici-pates in the process of ototoxicity induced by gentamicin and suggest that intervention aimed at autophagy may influence the ototoxicity process.
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