松质骨 OPG，RANKL 和 RANK 基因甲基化的相关性研究

摘要

Objective To study the relationship among OPG, RANKL, and RANK gene methylation in the cancellous bone. Methods A total of 8 patients, who had total hip arthroplasty from November 2011 to March 2012 in our department, were included.During the operations, the cancellous bone of the femoral neck was collected.The bone mineral density ( BMD) of the cancellous bone was detected.The methylation level of OPG, RANKL, and RANK gene was detected using methylated DNA immunoprecipitation-quantitative PCR method ( MeDIP-qPCR ) .The correlation analysis was also performed.Results The average BMD of the cancellous bone was 0.056g/cm3 .The average methylation level of OPG, RANKL, and RANK gene was 0.115%, 0.087%, and 0.079%, respectively.The methylation level of OPG gene was positively correlated with the mythylation level of RANKL and RANK gene (r=0.952, P<0.01;r=0.976, P<0.01;respectively).But no correlation between the ratio of OPG, RANKL gene and BMD of the cancellous bone was observed ( P>0.01 ) .Conclusion In the cancellous bone, the methylation level OPG gene methy lation level is positively correlated with the mehtylation levels of RANKL gene and RANK gene. But there is no apparent relationship between the mehtylation levels of OPG and RANKL gene and BMD of the cancellous bone.%目的：探求OPG，RANKL和RANK基因甲基化水平在松质骨组织中的相关性。方法选取2011年11月至2012年3月于我科进行人工髋关节置换患者8例，术中留取股骨颈内松质骨标本，进行标本的骨密度检测，并采用甲基化免疫共沉淀的方法检测标本OPG，RANKL和RANK基因的甲基化水平，进行相关性的统计学分析。结果该组患者股骨颈内松质骨骨密度均值为0．056 g/cm3，OPG基因甲基化率中值为0．115％，RANK和RANKL基因甲基化率分别为0．087％和0．079％。 OPG基因和RANKL基因甲基化率呈明显正相关（r＝0．952，P＜0．01），OPG基因和RANK基因甲基化率也呈明显的相关性，为正相关（r＝0．976，P＜0．01），但OPG基因和RANKL基因甲基化率的比值与标本的骨密度值没有明显的相关性（P＞0．01）。结论在松质骨组织中OPG基因甲基化水平分别和RANKL、RANK基因甲基化水平存在明显的正相关，但没有发现甲基化率比值与松质骨的骨密度存在明显的相关性。